顺铂对耳蜗单离听觉细胞─氧化氮合酶表达的影响  

Effects of Cisplation on NOS_Ⅱ Expression in Isolated Cochlear Auditory Cells

作　　者：曹恒明[1] 钱雪冶[2] 迟放鲁[2] 李华伟[2] 张勤修[2] 

机构地区：[1]江苏徐州医学院附属医院耳鼻咽喉科,徐州221002 [2]复旦大学医学院附属眼耳鼻喉科医院耳鼻咽喉科

出　　处：《听力学及言语疾病杂志》2001年第1期27-29,T002,共4页Journal of Audiology and Speech Pathology

摘　　要：目的 探讨顺铂对耳蜗单离听觉细胞诱导型─氧化氮合酶（ｉＮＯＳ／ＮＯＳⅡ）表达的影响。方法 以单 离毛细胞和单离螺旋神经节神经元为模型，用免疫组化方法（ＡＢＣ法）研究顺铂对细胞内ＮＯＳⅡ表达的影响。结果 在１ｍＭ顺铂平衡盐溶液中室温下培养两小时的外毛细胞及培养半小时的单离螺旋神经元与对照组相比显示了 对ＮＯＳⅡ抗体较强的免疫反应。结论 提示顺铂诱导了单离外毛细胞及单离螺旋神经元内ＮＯＳⅡ的合成增加，ＮＯ 可能参与了顺铂的耳毒作用。Objective To study the effects of cisplatin on the inducible nitric oxide synthase(iNOS/NOSⅡ) expression in isolated cochlear auditory cells. Methods Using immunohistochemisty (ABC method) to study the effects of cisplatin on the NOSⅡ expression in the model of lsolated hair cells and spiral neurons. ResuIts The NOSⅡ immunopositive staining was stronger in outer hair cells cultured in 1 mM cisplatin balanced salt solution for two hours at room temperature and in the spiral neurons cul- tured for half an hour than that of the contrast ones. Conclusion The results of this study indicate that cisplatin induced the high expression of NOSⅡ in isolated outer hair ceIIs and spiral neurons and suggest that NO may pIay some roles in the cisplatin ototoxi- city.

关 键 词：顺铂耳毒性 单离外毛细胞 单离螺旋神经元 诱导型一氧化氮合酶 

分 类 号：R764.5[医药卫生—耳鼻咽喉科]