葡萄卷叶病毒RT-PCR检测技术  

Detection of GLRaV by RT-PCR

作　　者：侯义龙[1] 

机构地区：[1]大连大学生物工程学院

出　　处：《植物保护学报》2003年第3期305-308,共4页Journal of Plant Protection

基　　金：国家自然科学基金(30170076);辽宁省博士起动基金(20021052)

摘　　要：为调查葡萄植株携带葡萄卷叶病毒(GLRaV)的情况,以带毒和非带毒指示植物“品丽珠”为试验材料提取总RNA,并以此总RNA为模板进行RT-PCR扩增,建立了GLRaV的RT-PCR检测体系,并对GLRaV cDNA序列进行测定,结果成功地检测到了GLRaV。经多次试验证明,该检测结果准确可靠,可以用于GLRaV的检测。The virus indicator plants Cabernet Franc were chosen as experimental material to establish the RT-PCR detection system for GLRaV. After the total RNA was extracted, the GLRaV was deteced by RT-PCR. The amplification of 300 bp cDNA sequence from GLRaV-infected plant demonstrated that the detection result is reliable.

关 键 词：葡萄 卷叶病毒 RT-PCR检测技术 逆转录-聚合酶链式反应 

分 类 号：S436.63[农业科学—农业昆虫与害虫防治]