流动注射化学发光法测定过氧化氢酶活性  

Determination of catalase activity by flow injection chemiluminescence method

作　　者：胡晓捷[1] 杨炜春[1] 童裳伦[1] 刘维屏[1] 

机构地区：[1]浙江大学环境科学研究所,浙江杭州310029

出　　处：《浙江大学学报（理学版）》2003年第6期678-680,共3页Journal of Zhejiang University（Science Edition）

基　　金：国家自然科学基金资助项目(39673420).

摘　　要：基于过氧化氢酶对Luminol-KIO4-H2O2体系化学发光反应的抑制作用,建立了流动注射化学发光快速测定过氧化氢酶的新方法.该方法线性2～200unit/L,检出限为0.7unit/L,对50unit/L过氧化氢酶10次平行测定的相对标准偏差为0.4%.用于土壤中过氧化氢酶活性的测定,与高锰酸钾滴定法相比,结果令人满意.A chemiluminescence suppression method for the determination of catalase activity based on Luminol-KIO_4-H_2O_2-catalase system was established. The linear range of the method for the determination of the catalase activity is 2~200 unit/L and the detection limit is 0.7 unit/L. The relative standard deviation(n=10) is 0.4% for 50 unit/L catalase. The method was used to determine the activity of catalase in soil with satisfactory results as compared with the standard method, potassium permanganate titration.

关 键 词：流动注射化学发光法 肥力测定 过氧化氢酶 活性 土壤 

分 类 号：S158.2[农业科学—土壤学]