金钱鱼凋亡相关基因SaAR对NIH-3T3细胞凋亡的影响  

Effects of Scatophagus argus apoptosis-related gene on apoptosis of NIH-3T3 cells

作　　者：欧阳平[1] 陈慧萍[2] 赖文岩[1] 杨文利[2] 徐安龙[2] 

机构地区：[1]第一军医大学南方医院心血管内科,广东广州510515 [2]中山大学生命科学学院,国家高技术计划海洋生物功能基因组开放实验室,广东广州510275

出　　处：《第一军医大学学报》2003年第11期1137-1138,共2页Journal of First Military Medical University

基　　金：国家863计划项目(2001AA628090)~~

摘　　要：目的观察金钱鱼凋亡相关基因SaAR(Scatophagusargusapoptosis related gene)对成纤维细胞增殖的影响。方法体外培养NIH-3T3细胞,应用脂质体将不同浓度的pcDNA3-SaAR真核表达质粒分别转染细胞并设立对照组进行比较,应用流式细胞术测定细胞凋亡率。结果SaAR基因可显著诱导NIH-3T3细胞凋亡,并呈现剂量依赖性(P<0.05)。结论SaAR基因对体外培养的血管外膜成纤维细胞凋亡有一定的诱导作用。Objective To observe the effects of Scatophagus argus apoptosis-related (SaAR) gene transfer on the apoptosis of NIH-3T3 cells. Methods A controlled observation of SaAR gene transfer (at different concentrations) into cultured NIH-3T3 cells via lipofectin was performed, and the rate of cell apoptosis was analyzed by flow cytomertry. Results The rate of cell apoptosis was (6.37±0.0.56)% in the control group, and was (11.89±1.13)%, (18.25±1.86)% and (22.34±1.08)% respectively in pcDNA3-SaAR gene groups corresponding to pcDNA3-SaAR gene concentrations of 2, 3 and 4 μg/ml respectively. It was shown that SaAR gene significantly induced fibroblast apoptosis when the concentration was above 2 μg/ml (P<0.05), in a dose-dependent manner as compared with the control group (P<0.05). Conclusion Transient transfection of SaAR gene can significantly induce apoptosis in NIH-3T3 cells.

关 键 词：金钱鱼 凋亡相关基因 SaAR NIH-3T3 细胞凋亡 流式细胞术 基因转移 成纤维细胞 血管阻塞性疾病 

分 类 号：R346[医药卫生—基础医学]