应用PCR快速制备细小病毒B19诊断芯片探针(英文)  

Quick preparations of human parvovirus B19 microarray probes using PCR

作　　者：吕梁[1] 马文丽[1] 王洪敏[1] 马晓冬[1] 孙朝晖[2] 郑文岭[2] 

机构地区：[1]第一军医大学分子生物学研究所,广东广州510515 [2]广州军区广州总医院肿瘤分子生物学研究所,广东广州510010

出　　处：《第一军医大学学报》2003年第11期1121-1124,共4页Journal of First Military Medical University

摘　　要：目的制备细小病毒诊断芯片探针。方法利用Primer Premier 5.0针对细小病毒B19基因保守区域设计PCR引物,将PCR产物克隆pMD-18 T载体。结果序列分析显示,PCR产物均为细小病毒B19特异保守基因。结论利用PCR扩增产物制备诊断芯片探针是一种简便有效的方法。Objective To prepare DNA microarray probes for the detection of human parvovirus B19. Method Specific PCR primers were designed with the Primer Premier 5.0 to amplify the conserved regions of human parvovirus B19 genome. The PCR products were cloned into the pMD-18 T vector. Result Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19. Conclusion PCR amplification of the conserved and specific human parvovirus B19 genes is simple and effective to prepare the desired probes.

关 键 词：PCR 制备 细小病毒B19 诊断芯片 探针 

分 类 号：Q819[生物学—生物工程]