禽劳斯肉瘤病毒群特异性抗原基因gp27片段的克隆及序列测定  

Cloning and sequence analysis of group specific antigen gp27 gene of rous sarcoma virus

作　　者：宋建臣[1] 关云涛[2] 刘怀然[2] 陈洪岩[2] 刘胜旺[2] 蔡雪辉[2] 夏长友[2] 

机构地区：[1]延边大学农学院动物医学系,吉林龙井133400 [2]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国兽医科技》2003年第11期21-24,共4页Chinese Journal of Veterinary Science and Technology

摘　　要：以RT PCR方法从人工感染禽劳斯肉瘤病毒 (Roussarcomavirus ,RSV )SR RSV的SPF雏鸡体内及SPF鸡胚成纤维细胞 (CEF)培养物中分别扩增出 72 0bp的 gp2 7片段 ,将RT PCR产物重组到质粒载体 pUC19中 ,并对重组质粒进行酶切分析及序列测定。结果表明 ,克隆片段为完整的目的基因 ,该片段的核苷酸序列与国外分离株RSVJ0 2 342的同源性达 97.3%。The gp27 gene of 720 bp was amplified by RT-PCR method from SPF chicken and Chicken Embyo Fibroblast (CEF) which were infected with Rous sarcoma virus (RSV), and the gp27 gene was cloned into a plasmid veetor pUC19, the recombined plasmid were analyzed by restriction enzyme digestion and sequence analysis, the result showed that the cloning gene was the full-length target gene which has a 97.7% homology with foreign isolate strains RSV(J02342).

关 键 词：鸡 禽劳斯肉瘤病毒 特异性抗原 gp27基因 基因克隆 序列测定 RT-PCR 

分 类 号：S852.659.3[农业科学—基础兽医学] S858.31[农业科学—兽医学]