抗PML抗体免疫荧光法快速诊断急性早幼粒细胞白血病  

Rapid diagnosis of acute promyelocytic leukemia by anti-PML antibody immunofluorescence assay

作　　者：秦亚溱[1] 喻新建[1] 刘艳荣[1] 陈珊珊[1] 

机构地区：[1]北京医科大学人民医院血液病研究所,北京100034

出　　处：《中国实验血液学杂志》1998年第1期76-78,共3页Journal of Experimental Hematology

摘　　要：对35例白血病病人骨髓标本进行抗PML抗体免疫荧光测定。19例急性早幼粒细胞白血病(APL)标本中,11例未治、3例服ATRA4天以内,以及3例复发病人均为典型APL荧光表现,2例ATRA诱导完全缓解APL病人荧光表现为非APL型。16例未治的非APL病人均为典型非APL表现。研究结果表明,抗PML抗体免疫荧光法简便、快速、特异,适于诊断未治和复发的APL病人。Bone marrow from 35 cases of leukemia was tested by anti-PML antibody immunofluorescence assay. A-mong 19 APL cases, 11 untreated, 3 cases treating with ATRA within 4 days and 3 relapsed cases exhibited characteristic APL-type fluorescent labelling pattern, 2 cases achieving complete remission by ATRA induction exhibited non-APL type pattern. All 16 non-APL cases exhibited non-APL type labelling pattern. These results suggested that the rapid, simple and specific assay could serve as an useful diagnosis tool for untreated and relapsed APL patients, but it is not suitable for detecting minimal residual disease.

关 键 词：抗PML抗体免疫荧光法 急性早幼粒细胞白血病 PML蛋白 PML/RARa融合蛋白 

分 类 号：R446.6[医药卫生—诊断学] R733.7[医药卫生—临床医学]