外源性三磷酸腺苷和腺苷对肿瘤细胞增殖的影响  

Effects of extracellular adenosine triphosphate and adenosine on tumor cell proliferation

作　　者：王明霞[1] 任雷鸣[1] 单保恩[2] 

机构地区：[1]河北医科大学药学院,石家庄050017 [2]河北医科大学第四医院,石家庄050011

出　　处：《中国药理学通报》2003年第11期1242-1246,共5页Chinese Pharmacological Bulletin

基　　金：国家人事部留学回国人员科技活动择优资助项目 ;No 2 0 0 2 2 (河北 )

摘　　要：目的　研究三磷酸腺苷 (ATP)和腺苷 (ADO)对人红白血病细胞株K5 6 2、人胃中分化腺癌细胞株HGC 2 7、人食管低分化鳞癌细胞株TE 13细胞增殖的影响。方法　采用MTT法测定ATP和ADO抑制肿瘤细胞增殖的作用 ,Wright’s Giemsa染色观察细胞形态学的改变。结果　在 0 0 1～ 1 0mmol·L-1浓度范围内 ,ATP和ADO可不同程度地抑制TE 13、HGC 2 7和K5 6 2细胞的增殖 ,其中对TE 13的作用最强 ;ATP和ADO作用 72h后 ,对TE 13细胞的抑制率分别为80 5 2 %和 74 0 3%。细胞经高浓度 (1mmol·L-1)的ATP和ADO处理后 ,细胞形态出现了凋亡的特征。结论　ATP抗TE 13和HGC 2 7肿瘤细胞增殖的作用与其代谢产物ADO部分相关 。AIM To study the growth inhibition of adenosine triphosphate (ATP) and adenosine (ADO) on cultured tumor cells in vitro. METHODS MTT assays were used to determine the inhibition of proliferation of ATP and ADO on several tumor cell lines, including human squamous esophageal carcinoma cells TE-13, human stomach carcinoma cells HGC-27 and human erythroleukemia cells K562. The morphological changes of ATP and ADO on the cell lines were observed under light microscope. RESULTS ATP and ADO produced a certain inhibition effect on TE-13, HGC-27, K562 cells at different concentration between 0.01～1.0 mmol·L -1. The maxium inhibition fraction of TE-13, HGC-27, K562 cells exposed to ATP for 72 h was 80.52%, 58.67% and 45.07%, respectively; and to ADO for 72 h, was 74.03%, 52% and 30.99%, respectively. Under light microscope, the tumor cells exposed to higher concentration(1 mmol·L -1) of ATP and ADO displayed morphological changes of apoptosis. CONCLUSION These results suggested that ATP has a certain cytotoxic effect on several tumor cell lines, it might partly be related to ADO. Its mechanism might involve apoptosis.

关 键 词：三磷酸腺苷 腺苷 抗肿瘤作用 细胞凋亡 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]