冬凌草乙素诱导GBC-SD细胞的凋亡及对Bcl-2、p53、Fas/APO-1、C-myc表达的影响  

Effect of rubescensine B on apoptosis and Bcl-2、p53、Fas/APO-1、C-myc expression in GBC-SD cells

作　　者：薛宏伟[1] 潘祥麟[2] 

机构地区：[1]青岛大学医学院附属医院肿瘤科,青岛266003 [2]山东大学第二医院血液肿瘤科,济南250033

出　　处：《中国药理学通报》2003年第11期1247-1251,共5页Chinese Pharmacological Bulletin

基　　金：山东省中医药管理局资助项目 ;No 2 0 0 10 0 9

摘　　要：目的　探讨冬凌草乙素对人胆囊癌GBC SD细胞增殖抑制和诱导调亡的机制。方法　不同浓度的冬凌草乙素处理GBC SD细胞后 ,用MTT法检测GBC SD细胞的存活率 ,通过倒置显微镜、扫描电子显微镜、流式细胞仪、荧光分光光度计观察和分析凋亡细胞 ,用免疫组织化学法和流式细胞仪检测细胞内Bcl 2、p5 3、Fas/APO 1、C myc的表达。结果　冬凌草乙素浓度依赖性地抑制GBC SD细胞增殖及诱导其凋亡 ;药物作用 2 4h后 ,细胞内Bcl 2、p5 3、Fas/APO 1、C myc的表达有不同程度的下调 ;Caspase 3活性与凋亡程度相关。结论　冬凌草乙素抑制GBC SD细胞的增殖和诱导其凋亡与Bcl 2、p5 3、Fas/APO 1、CAIM To investigate the inhibition and apoptosis mechanism of GBC-SD cells induced by rubescensine B. METHODS Using MTT, convert microscopy, electron microscopy, flow cytometry, an immunohistochemical assay, and spectrofluorometry demonstrate the presence and pathogenesis of apoptosis after treated by rubescensine B. RESULTS After exposure to Rubecensine B GBC-SD cells were induced to apoptosis in dose-dependent manner, and the level of Bcl-2,p53,C-myc,Fas/APO-1 were decreased within 24 hours, reversely the activity of Caspase-3 was enhanced with the appearance of apoptosis. CONCLUSION Rubecensine B can induce GBC-SD cells apoptosis related to Bcl-2,p53,Fas/APO-1 and C-myc.

关 键 词：冬凌草乙素 DBC-SD 凋亡 BCL-2 P53 FAS/APO-1 C-myc 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]