日本血吸虫重组信号蛋白14-3-3疫苗免疫保护性的观察  

Protective Immunity Induced by Recombinant Signaling Protein 14-3-3 Vaccine of Schistosoma japonicum

作　　者：刘庆中[1] 沈继龙[1] 

机构地区：[1]安徽医科大学病原生物学教研室

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第5期257-260,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金 (No.30 1 70 841 );安徽省自然科学基金(No .0 0 4 4 547)资助项目~~

摘　　要：目的　研究日本血吸虫 (中国大陆株 )重组信号蛋白 14 3 3 (rSj14 3 3 )作为血吸虫病疫苗分子的潜能 ,并探讨rSj14 3 3、rSjGST两种重组蛋白作为疫苗的协同作用及结核杆菌低分子量耐热多肽 (Mtb)激活的γδ T细胞在抗血吸虫病中的作用。　方法　用SDS PAGE、电洗脱和透析的方法制备rSj14 3 3和rSjGST抗原 ,将两种抗原 (分别用福氏佐剂和Mtb为佐剂 )分别免疫BALB/c小鼠后 ,进行尾蚴攻击感染实验。在攻击感染 6wk后 ,剖杀小鼠计算各组的减虫率。　结果　各组的减虫率为rSj14 3 3 +福氏佐剂组 3 2 .2 0 % ,rSj14 3 3 +rSjGST +福氏佐剂组 3 1.10 % ,rSj14 3 3 +Mtb佐剂组 2 7.96% ,rSj14 3 3 +rSjGST +Mtb佐剂组 2 6.0 0 % ,rSjGST +Mtb佐剂组 2 7.10 % ;各组的减卵率分别为 (按以上组序 ) 5 0 .40 %、5 3 .3 0 %、5 1.10 %、5 8.60 %和 5 1.3 0 %。　结论　rSj14 3 3具有一定的抗血吸虫潜能 ,有可能成为抗日本血吸虫疫苗 ,但未见rSj14 3 3和rSjGST的协同作用 ;Mtb激活扩增的γδ T细胞在抗血吸虫免疫中的效果与福氏佐剂产生的免疫作用类似。Objective To evaluate the immunoprotective effect of Schistosoma japonicum (Chinese strain) recombinant signaling protein 14-3-3(rSj14-3-3), and to observe the synergism of rSj14-3-3 and rSjGST proteins as candidate vaccine and the effect of γδ-T cells activated by Mtb against Schistosoma japonicum. Methods BALB/c mice immunized with rSj14-3-3 and rSjGST purified through SDS-PAGE, electroelution and dialysis were challenged by cercaria infection. Six weeks after challenging infection, the mice were killed and the worm and egg reduction rates were calculated. Results Worm reduction rate was found to be 32.20% in rSj14-3-3+Freund adjuvant group, 31.10% in rSj14-3-3+rSjGST+Freund adjuvant group, 27.96% in rSj14-3-3+Mtb group, 26.00% in rSj14-3-3+rSjGST+Mtb group, and 27.10 % in rSjGST+Mtb group, respectively, number of eggs in liver tissue was reduced by 50.40%, 53.30%, 51.10%, 58.60% and 51.30%, respectively. Conclusion rSj14-3-3 could induce partial immunity against Schistosoma japonicum in BALB/c mice, and might serve as a candidate vaccine; γδ-T cell activated by Mtb played a role in anti-Schistosoma japonicum similar to the immune reactions induced by Freund adjuvant, but no synergistic effect combined with rSjGST was observed.

关 键 词：日本血吸虫 重组信号蛋白 14-3-3 疫苗 免疫保护性 观察 协同作用 γδ-T细胞 

分 类 号：R383.24[医药卫生—医学寄生虫学]