核糖体蛋白S7在大劣按蚊抗疟原虫感染免疫研究中的内标作用  

The Internal Control Role of Ribosomal Protein S7 in the Defense of Anopheles dirus Against Plasmodium Infection

作　　者：徐文岳[1] 黄复生[1] 段建华[1] 

机构地区：[1]第三军医大学基础部病原生物学教研室,重庆400038

出　　处：《中国寄生虫学与寄生虫病杂志》2003年第5期264-267,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金资助项目 (No .30 2 0 0 2 37)~~

摘　　要：目的　探讨核糖体蛋白S7(ribosomalprotein ,rpS7)在大劣按蚊抗约氏疟原虫感染免疫中的作用。　 方法　根据rpS7的保守mRNA序列设计简并引物 ,以大劣按蚊血细胞mRNA为模板 ,进行RT PCR扩增 ,克隆、并测定序列 ;RT PCR结合凝胶图像扫描比较、分析血餐后d1、d2、d3、d4、d7和d11,未吸鼠血组 (N)、吸正常鼠血组 (B)和吸感染约氏疟原虫鼠血组 (I)大劣按蚊血细胞中rpS7的转录差异。　 结果　成功克隆大劣按蚊rpS7部分cDNA序列 ( 4 65bp) ,吸血和约氏疟原虫感染均未明显地影响大劣按蚊血细胞中rpS7的转录 (P >0 .0 5 )。　 结论　大劣按蚊血细胞rpS7可作为研究大劣按蚊抗约氏疟原虫感染免疫相关因子的内参照。Objective To investigate the role of ribosomal protein S7(rpS7) in the defense of Anopheles dirus against infection. Methods rpS7 was amplified from Anopheles dirus hemocytes with degenerated primers designed according to the conservative region of S7, rpS7 was then cloned using T/A cloning kit and the inserted fragment was sequenced. The difference of the transcript abundance of rpS7 from Anopheles dirus hemocyte among non-blood-fed (N), normal-blood-fed (B) and Plasmodium yoelii infected groups (I) was also analyzed by RT-PCR and gel scanning system at d1, d2, d3, d4, d7 and d11 after blood feeding. Results There is no significant difference of rpS7 signal between the three groups. Conclusion Anopheles dirus S7 can be used as an internal control to study the role of Anopheles dirus related immune factors in Plasmodium infection.

关 键 词：核糖体蛋白S7 大劣按蚊 抗疟原虫感染 免疫研究 内标作用 

分 类 号：R384.1[医药卫生—医学寄生虫学]