高效毛细管电泳同时分析尿中20种碱性药物与毒物  

Analysis of twenty basic drugs in urine by high performance capillary electrophoresis

作　　者：金琦芸[1] 梁晨[1] 郭幼梅[1] 张玉荣 张润生 龚飞君 

机构地区：[1]复旦大学药学院药物分析教研室,上海200032 [2]上海刑事科学技术研究所,上海200083

出　　处：《中国临床药学杂志》2003年第6期343-346,共4页Chinese Journal of Clinical Pharmacy

摘　　要：目的 :建立用固相萃取高效毛细管电泳同时分离分析尿中 2 0种碱性药物与毒物的方法。方法 :尿样以oasis小柱提取 ,以多沙普伦为内标 ,采用未涂层毛细管 ( 75 μmID× 5 0 2cm ,有效分离长度为 4 0cm) ,缓冲液为 15 0mmol·L-1磷酸盐 + 2 0%甲醇 (pH =2 38) ,压力进样 8s,运行电压 16kV ,检测波长 2 0 0nm。结果 :本法分离效率高 ,药、毒物最低检测浓度为 15～ 80μg·L-1;线性范围 6 0～ 5 0 0 0 μg·L-1,日内、日间RSD <12 %。结论 :本法可用于多种类别的药、毒物的分析 ,增加检出几率 ,减少漏检。AIM:To develop a solid phase extraction high performance capillary electrophoresis method for simultaneous determination of twenty basic drugs in urine METHODS: With the doxapram as internal standard, Oasis column was used to extract drugs from urine The separation was performed on a fused silica capillary of 75 μm ID × 50 2 cm (40 cm of effect length) The running buffer was 150 mmol·L -1 phosphate + 20 % methanol (pH=2 38) Sample solution was injected with pressure mode and the running voltage was 16 kV The detection wavelength was set at 200 nm RESULTS:The linear ranges of the calibration curves were 60 5 000 μg·L -1 and detection limits were 15 80 μg·L -1 The RSD of intra day and inter day was less than 12 % CONCLUSION:The method is effective,simple and reliable The twenty basic drugs of different kinds can be separated at the same time This method has been used in real case of intoxication

关 键 词：高效毛细管电泳 尿 碱性药物 毒物 固相萃取 

分 类 号：R96[医药卫生—药理学]