黑脊倒刺鲃精巢结构和精子发生的研究  

STUDIES ON TESTICULAR HISTOLOGY AND SPERMATOGENESIS OF TELEOST,SPINIBARBUS CALDWELLI (NICHOLS)

作　　者：林丹军[1] 尤永隆[1] 苏敏[1] 

机构地区：[1]福建师范大学生物学系,福州350007

出　　处：《水生生物学报》2003年第6期563-571,共9页Acta Hydrobiologica Sinica

基　　金：福建省科技厅项目 (K2 0 0 0 91)资助

摘　　要：用光学显微镜和电子显微镜研究了黑脊倒刺精巢的组织结构和精子发生过程。精巢属于小叶型 ,由精小叶、小叶间质、壶腹腔和输出管构成。精小叶由各期生精细胞和支持细胞构成。除初级精原细胞以外的各期生精细胞和支持细胞组成了精小囊。每一精小囊中的生精细胞发育同步。成熟的精子从精小囊中释放出来 ,进入小叶腔中。在精巢的腹侧 ,小叶腔与壶腹腔连接。在壶腹腔的外侧 ,有一条与壶腹腔平行的输出管。壶腹腔与输出管相通。在壶腹腔和输出管中都充满精子。精巢的后端与贮精囊相连。贮精囊中充满形状不规则的腔隙。腔隙中有精子分布。输出管从精巢延伸出来 ,进入贮精囊中 ,位于贮精囊的一侧。左右两个贮精囊通向一条共同的输精管。输精管上皮具有分泌功能。精子发生在精小叶中进行。精子发生经历了初级精原细胞、次级精原细胞、初级精母细胞、次级精母细胞和精子细胞阶段。精子细胞经过精子形成过程 ,形成精子。Spinibarbus caldwelli (Nichols) belongs to the family Cyprinidae, Barbinae.It is now becoming an important fish for aquaculture in China. The testicular histology and spermatogenesis of the fish have not been reported. In May and June 2001,the testes of 5 male Spinibarbus caldwelli were sampled for histological observation under both light microscope and transmission electron microscope. The testis was lobular type,which was typical of most teleosts. It consisted of seminiferous lobuli, interlobular septum,cavum ampulla and vas efferens.In the outer layer of the testicular cortex,the seminiferous lobuli arranged radially,similar to that of the order Perciformes.However in the inner layer of the cortex,the distribution of the seminiferous lobuli was more irregulary. That was similar to those in most fishes of Cyprinidae. The seminiferous lobulus consisted of various stages of spermatogenic cells and Sertoli cells .The spermatogenic cells consisted of primary spermatogonia, secondary spermatogonia, primary spermatocytes ,secondary spermatocytes and spermatids.The primary spermatogonia distributed singly.Besides primary spermatogonia ,other stages of spermatogenic cells were in the spermatogenic cysts. The wall of the spermatogenic cysts was made of Sertoli cells. Within a spermatogenic cyst,all the spermatogenic cells were at the same developmental stage.Mature sperms were released from the cysts and trnasfered into the lobular lumen.Whitin the cytoplasm of the Sertoli cells ,there were lots of electron dense granules.One of the important functions of the Sertoli cells was to engulf and eliminate degenerated spermatids.According to the morphyology of the granules and spermatogonia's sleeves and flagella existing in the cytoplasm of the Sertoli cells ,the granules might be the nuclei of the spermatids, which were engulfed by the Sertoli cells. All the lobular lumen converged at the ventral side of the testis, and formed cavum ampulla,which was at the orientation from anterior end to the posterior end of the tes

关 键 词：精巢 组织结构 精子发生 黑脊倒刺鲃 鲤科 鱼巴亚科 

分 类 号：Q959.468[生物学—动物学]