GPV野毒株的分离及PCR检测方法的应用  

Isolation of GPV wild strain and detection by PCR

作　　者：布日额[1] 王君伟[1] 吴金花[2] 田丽红[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]内蒙古民族大学动物科技学院,内蒙古通辽028042

出　　处：《中国预防兽医学报》2003年第6期469-472,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省"十五"攻关课题 (合同号GB0 1B5 0 2 0 2 )

摘　　要：在本实验中 ,用根据CPVHl标准毒核苷酸VP1_VP3和VP3两个区段基因序列设计的 2对特异性引物GRPl/GRP2和GFl/GF2 ,用该 2对引物对从鹅细小病毒野毒感染的病鹅分离的GPV野毒株进行PCR检测 ,结果 2对引物GRPl/GRP2和GFl/GR2 ,均能特异性地扩增出与预期片段大小相符的 0 .6bp和 1.6bp两个片段。回归试验的结果表明 ,该GFV野毒株的感染潜伏期为 8d ,病程为 1～ 3d ,致死率达 10 0 %。In this assay,two pairs of primers were designed according with DNA sequence from GPV Hl standard strain.Using the two pairs of primers GRPl/GRP2 and GFl/GR2 detecting the GPV wild strain isolated from infected gooses by PCR.The result showed that both of the two pairs of primers GRPl/GRP2 and GFl/GR2 could specifically amplified expected two bounds of 0.6 bp and 1.6 bp.The results of regressive assays showed that latent period was 8 d,lasting 1～3 d after infected,lethal rate was 100 % of GPV wild strain infected.

关 键 词：鹅细小病毒 野毒株 PCR检测 

分 类 号：S852.659.2[农业科学—基础兽医学]