PCR和核酸探针技术诊断MD和RE的比较研究  

Comparison of PCR and dot-hybridization for the diagnosis of MD and RE

作　　者：王桂军[1] 韦平[1] 李康然[1] 左天荣[1] 刘岳龙[2] 金文杰[2] 秦爱建[2] 

机构地区：[1]广西大学养禽与禽病研究所 [2]扬州大学畜牧兽医学院,江苏扬州225009

出　　处：《中国预防兽医学报》2003年第6期479-482,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：教育部科技重点研究项目 (项目号 :0 2 116 );江苏省高校重点实验室开放课题 (KJS0 10 5 7);广西"十百千人才"工程基金项目 (992 15 );广西九.五科技攻关项目 (99110 4 )

摘　　要：分别应用聚合酶链反应 (PCR)技术和核酸探针的点杂交技术 ,对临床鸡肿瘤 /可疑肿瘤病料分别进行马立克氏病 (MD)和禽网状内皮增生症 (RE)的检测。结果MD和RE的PCR检测阳性率分别为 81.9%和 5 3.3% ,探针点杂交检测的阳性率则分别为 77.1%和 2 7.1%。研究的结果表明 ,两种检测技术都有很高的特异性 ,相比而言PCR技术检测的敏感性更高 ,而且更快速、操作更简便、成本更低。PCR and dot-hybridization were used respectively to detect the tumor samples from the field in Guangxi for Marek's disease (MD) and reticuloendoepithiosis (RE). The positive rate of 81.9 % and 53.3 % were obtained respectively for MD and RE by PCR, while those of 77.1 % and 27.1 % were obtained respectively by dot-hybridization. The results of the comparisons between the two techniques demonstrated that the developed PCR-based protocol has the advantages in the aspects of sensitivity, rapidity, labor and expense over the dot-hybridization technique..

关 键 词：马立克氏病 禽网状内皮增生症 聚合酶链反应 核酸探针 比较 

分 类 号：S852.65[农业科学—基础兽医学] Q78[农业科学—兽医学]