鸡传染性支气管炎病毒LH2/01/10的分离鉴定  

Isolation and identification of an avian infectious bronchitis virus strain LH2/01/10

作　　者：刘丽玲[1] 孔宪刚[1] 刘胜旺[1] 刘玉芬[1] 谷守林[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国预防兽医学报》2003年第6期447-450,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：黑龙江省政府博士后科研启动金项目 (LRB_KY0 10 4 5 )

摘　　要：20 0 1年在我国黑龙江省某鸡场疑似鸡传染性支气管炎的发病鸡群中 ,分离到一株病毒 ,将该病毒接种 10日龄SPF鸡胚 ,取 72h的尿囊液进行电镜观察并用 1%胰酶处理 ,结果发现尿囊液中存在典型的冠状病毒 ,用胰酶处理过的尿囊液能凝集SPF鸡的红细胞 ,初步鉴定为鸡的传染性支气管炎病毒。将该病毒尿囊液再次接种 10日龄SPF鸡胚 ,通过病毒对鸡胚的致病作用、病毒超微形态特征以及病毒凝集鸡红细胞的特性等对该毒株进行研究 ,结果表明 :经胰酶处理后的病毒尿囊液可凝集鸡红细胞。鸡胚的第二代病毒尿囊液 (命名为LH2 / 0 1/ 10 )分别接种 1日龄和 15日龄的SPF鸡 ,发现对不同日龄的鸡表现不同的致病性 ,对 1日龄接种鸡和同笼饲养的同居对照鸡致病力高 ,发病率为 11/ 11,致死率分别为 4 / 6和 2 / 5 ;15日龄接种和同居感染鸡发病率为 9/ 9,致死率分别为 1/ 5和 1/ 4。实验应用反转录_聚合酶链式反应技术对LH2 / 0 1/ 10的膜蛋白基因进行扩增、克隆和序列测定 ,结果表明该基因具有IBVM基因的共有分子特征 ,与IBV标准株M4 1的M基因核苷酸的同源性为 90 % ,氨基酸的同源性为 91%。这从分子水平进一步证实引起鸡群死亡的病毒为鸡传染性支气管炎病毒。A field strain of Infectious Bronchitis Virus (IBV) was isolated from immuned chicken trachea of the affected young layer. Biological characteristics of the virus were studied by virus pathogenicity to SPF chicken embryos and SPF chickens,morphological observation by electron-microscope and hemagglutination activity of virus to chicken red blood cells. It was demonstrated that the virus showed typical shape shared by members of Coronaviridae under electronic microscope and could hemagglutinate chicken red blood cells after treatment with 1 % trypsin. The gross lesions caused by the virus to SPF chicken embryos and SPF chickens were obvious. The morbidity of the second passage virus (LH2/01/10) to 1-day-old chickens was 11/11 and mortality was 4/6,2/5 to challenged chickens and chickens living in common and to 15-day-old chickens was 9/9 and 1/5,1/4 respectively. In addition, M gene of LH2/01/10 shared the typical molecular characteristics of all IBV M genes.

关 键 词：传染性支气管炎病毒 分离鉴定 

分 类 号：S858.31[农业科学—临床兽医学]