伪狂犬病病毒囊膜蛋白ISCOMs免疫原性测定  

The immunogenicity of ISCOMs containing envelope proteins of pseudorabies virus in mice

作　　者：陈少莺[1] 胡奇林[1] 程晓霞[1] 俞伏松[1] 陈仕龙[1] 林天龙[1] 程由铨[1] 林锋强[1] 朱小丽[1] 

机构地区：[1]福建省农科院畜牧兽医研究所,福建福州350013

出　　处：《中国预防兽医学报》2003年第6期458-461,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：福建省自然科学基金资助项目 (编号 :B0 0 10 0 2 9)

摘　　要：应用伪狂犬病病毒囊膜蛋白与QuilA结合制备囊膜蛋白免疫刺激复合物 (PRV_ISCOMs) ,并通过ELISA、血清中和试验和淋巴细胞转化试验 (Brdu_ELISA法 )测定其诱导小鼠体液和细胞免疫应答水平。结果如下 :1)小鼠分别接种3、7、10ug的PRV_ISCOMs后 ,于接种后PI 7d血清中可测出ELISAIgG抗体 ,随后抗体水平逐渐升高。间隔 2 1d加强免疫后 ,ELISAIgG抗体水平进一步提高 ,且中和抗体效价均在 1∶2 2以上 ,而未加佐剂的囊膜蛋白对照组均无中和抗体 ;2 )淋巴细胞转化试验初步结果表明PRV_ISCOMs能诱导小鼠的细胞免疫应答。 3)免疫保护力测定显示免疫鼠能抵抗强毒攻击。上述结果表明PRV_ISCOMs具有良好的免疫原性 ,能诱导小鼠的体液免疫和细胞免疫应答。The PRV immunostimulating complexes (PRV-ISCOMs) were prepared by integrating envelope proteins of pseudorabies virus(PRV) into QuilA matrix, and its immunogenicity in mice was detected by ELISA,Neutralization test (NT) and proliferation of lymphocyte test (Brdu-ELISA assay). The results indicated: 1)The mice inoculated with 3, 7, 10ug of PRV-ISCOMs respectively developed ELISA IgG antibody 7 days post-inoculation, and had better antibody response after second inoculation interval 21 days. The neutralizing antibody titer (PD50)of mice after second inoculation reached above 1:22 and that of controls was negative. 2) The results of Brdu-ELISA indicated primarily that the mice vaccinated with PRV-ISCOMs can induce stronger cellular immune response than the controls. 3) The mice inoculated with PRV-ISCOMs obtained complete protection when challenged with PRV- FA 20 days after second inoculation. These results showed that PRV-ISCOMs can elicited excellent humoral and cellular immune response.

关 键 词：伪狂犬病病毒 囊膜蛋白 免疫刺激复合物 免疫原性 

分 类 号：S858.28[农业科学—临床兽医学] S852.42[农业科学—兽医学]