神经干细胞诱导骨髓基质细胞向神经元样细胞分化  

Bone marrow stromal cells induced by neural stem cells to differentiate into neuron-like cells

作　　者：杨立业[1] 刘相名[2] 郑佳坤[1] 惠国桢[2] 郭礼和[3] 

机构地区：[1]广东省潮州市中心医院神经外科,广东潮州521021 [2]苏州大学附属第一医院神经外科,江苏苏州512006 [3]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031

出　　处：《中华神经医学杂志》2003年第6期407-409,共3页Chinese Journal of Neuromedicine

基　　金：广东省自然科学基金(012452;020001)

摘　　要：目的 研究骨髓基质细胞向神经元样细胞分化的条件。方法 神经干细胞诱导骨髓基质细胞向神经元样细胞分化,免疫细胞化学方法对分化的和未分化的细胞进行鉴定。结果 神经干细胞可诱导骨髓基质细胞向神经元样细胞分化,分化的细胞表达神经元的标志物神经元特异性烯醇化酶(NSE)。结论 骨髓组织中存在能分化为神经元的干细胞,可能成为中枢神经系统自体移植的干细胞的来源。Objective To investigate the conditions of inducing bone marrow stromal cells to neuron-like cells. Methods Neural stem cells were labeled by adenoviral carrier Ad5 gal and were adapted to induce BMSCs to differentiate. Undifferentiated cells and differentiated cells were identified with immunocytochemistry. Results Neural stem cell induced BMSCs to differentiate into neuron-like cells. They exhibited a neuronal phenotype and expressed neuron-specific enolase (NSE). Conclusion The data support the hypothesis that adult bone marrow stem cells is capable of differentiating into neuron-like cells, which may represent an alternative autologous stem cell source for CNS cell transplantation.

关 键 词：神经干细胞 骨髓基质细胞 神经元 中枢神经系统 BMSCS 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]