GABA_A受体拮抗剂对吸入麻醉药催眠作用的影响  

A study on the analeptic action of GABA_A receptor antagonist in anesthetized mice

作　　者：颜梅[1] 戴体俊[2] 

机构地区：[1]徐州医学院药理学教研室,江苏徐州221002 [2]徐州医学院麻醉学系,江苏徐州221002

出　　处：《徐州医学院学报》2003年第6期506-508,共3页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金资助项目 (3 9970 715);江苏省自然科学基金资助课题 (BK2 0 0 114 3 )

摘　　要：目的　分析吸入麻醉药乙醚、安氟醚、异氟烷和七氟醚催眠作用与GABAA 受体的关系。方法　给小鼠分别腹腔注射麻醉剂量的乙醚、安氟醚、异氟烷、七氟醚 ,待小鼠翻正反射消失后 1min ,静脉注射一叶秋碱 5mg/kg或侧脑室注射荷包牡丹碱每只 0 .5μg /10 μl,对照组分别注射同等容积的生理盐水或人工脑脊液 ,观察并记录各组的翻正反射消失持续时间。结果　一叶秋碱和荷包牡丹碱对上述 4种麻醉药的翻正反射消失持续时间均无明显影响 (P >0 .0 5)。结论　GABAA 受体并非乙醚、安氟醚。Objective To study the r elationship between the volatile anesthetics and GABA A receptor.?M ethods Anesthetic ether (1.55 ml/kg), enflurane (3 ml/kg), isofluran e (2 ml/kg) or sevoflurane (6.5 ml/kg) was administered i.p. in mice. One min af ter the disappearance of righting reflex, securinine (5 mg/kg, iv) or bicucullin e (0.5 μg/10 μl, icv) was injected. An equal volume of normal saline or artifi cial cerebral spinal fluid was given in control group. The recovery time of righting reflex was recorded after the second injection.?Results The recovery time of righting reflex was not reduced in the securinin e and bicuculline test groups.?Conclusion GABA A recep tors are not the major target loci of volatile anesthetics studied.

关 键 词：GABAA受体拮抗剂 吸入麻醉药 催眠 一叶秋碱 荷包牡丹碱 

分 类 号：R614[医药卫生—麻醉学] R971[医药卫生—外科学]