定向进化人α型干扰素基因家族的研究  

Study on the Directed Evolution of Interferon Family

作　　者：段招军[1] 王红艳[1] 李武平[1] 吕宏亮[1] 赵军[1] 魏开坤[1] 马学军[1] 侯云德[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室,北京100052

出　　处：《病毒学报》2003年第4期301-305,共5页Chinese Journal of Virology

摘　　要：应用DNA改组(DNAshuffling)技术重组了12种人α型干扰素基因,并结合噬菌体表面呈现(phagedisplay)技术构建了噬菌体干扰素改组文库,采用简便的、功能性的定向竞争筛选方法,获得了比活达1 0×109IU/mg的新型α型基因工程复合干扰素,是目前国际上已投产的α2型干扰素的5倍,具有生产应用前景,上述策略也为其它细胞因子或酶的改造提供了借鉴方法。This is the report of dir ected evolution of Human Interfero n Alpha Genes using DNA shuffling combined with phage display techno logy and cell-based competitive sc reening methods.The novel interfer on alpha hybrid with improved bioa ctivity was obtained and shows a g reat prospect in production.This s trategy also offers some informati on on the way of directed evolutio n of other cytokines or proteins.

关 键 词：DNA改组 噬菌体表面呈现技术 干扰素 表达 纯化 

分 类 号：Q786[生物学—分子生物学]