重组人胰岛素的纯化及其性质的初步研究  

Research on the purification and characterization of the recombinant human insulin

作　　者：翁樑[1] 刘丽军[1] 王智[1] 刘丹[1] 谢晓娜[1] 周秋丽[1] 刘嵬[2] 苟小军[2] 

机构地区：[1]吉林大学分子酶学工程教育部重点实验室,长春130023 [2]成都大学分子进化工程实验室,成都610106

出　　处：《生物技术通讯》2003年第5期387-389,共3页Letters in Biotechnology

摘　　要：构建含有人胰岛素原基因的重组载体,并在大肠杆菌表达系统中进行高效表达。表达产物经变性、复性、凝胶过滤纯化后,再经胰蛋白酶和羧肽酶B酶切作用,产物经离子交换层析纯化得到重组人胰岛素,且具有天然生物学活性。The recombinant plasmid hPI /pBV220was constructed successfully,transformed into E.coli DH5αand ex-pressed in E.coli with high level,the expression product was denatured,renatured and purified by Sephacryl S-100gel filtration.The purified hPI was digested with typsin and carboxypeptidase B and purified by DEAE Sepharose Fast Flow.The purified recombinant human insulin with natural biological activity were obtained.

关 键 词：人胰岛素 纯化 受体结合活性 重组载体 

分 类 号：Q78[生物学—分子生物学] Q578