三种中药处方对SARS相关冠状病毒体外抑制作用的初步研究  

Inhibitory effects of three prescriptions of traditional Chinese medicine on SARS-associated coronavirus in vitro

作　　者：朱舜亚[1] 李晓萸[1] 魏云玲[1] 杨佩英[1] 秦鄂德[1] 

机构地区：[1]军事医学科学院微生物流行病研究所,北京100071

出　　处：《生物技术通讯》2003年第5期390-392,共3页Letters in Biotechnology

摘　　要：探讨三种中药处方对Vero-E6细胞内SARS相关冠状病毒的抑制作用。取一定量的SARS相关冠状病毒BJ01株加入培养好的Vero-E6细胞中,置37℃、5%CO2孵箱吸附2h,吸出病毒液,再分别加入不同浓度的药物稀释液,在同样条件下培养48～72h,观察细胞病变情况。迪康注射液、连花清瘟胶囊和复方连蒲颗粒抑制Vero-E6细胞内SARS-CoV的半数有效浓度EC50分别为0.056、0.11和0.49mg/ml,治疗指数TI分别为53.6、40.3和4.0。说明上述三种中药处方在体外对SARS-CoV有一定抑制作用,To observe the inhibitory effects of prescriptions of traditional Chinese medicine on growth of SARS-CoV in vitro.SARS-CoV strain BJ01were added on confluent layers of Vero-E6cells in96-well microplates,which were incubated at37℃in5%CO 2 incubator for2h.After adsorption,the cultured medium was thrown away and the different concentrations of prescriptions were added respectively.Then,the culture microplates were incubated for48-72h under the same conditions,and the cytopathogenicity induced by the virus were scored.The50%effective concentration(EC 50 )of Di-Kang injection,Lian-Hua-Qing-Wen capsule and Fu-Fang-Lian-Pu pellet was0.056,0.11and0.49mg /ml,respectively,and the treatment index(TI)of them was53.6,40.3and4.0,respectively.Three pre-scriptions of traditional Chinese medicine mentioned above were proved to have inhibitory effects on the SARS-CoV cultured in Vero-E6cells.

关 键 词：SARS 冠状病毒 药物筛选 中药处方 

分 类 号：R28[医药卫生—中药学] R373.1[医药卫生—中医学]