再障和特发性血小板减少性紫癜患者血浆血小板生成素含量研究  

Plasma thrombopoietin levels in patients with aplastic anemia and idiopathic thrombocytopenic purpura

作　　者：徐瑞容[1] 顾静[1] 陆璐[2] 陈秀芳[1] 

机构地区：[1]南通医学院附属医院血液研究室,南通226001 [2]南通医学院组织胚胎学教研室

出　　处：《南通医学院学报》2003年第4期388-389,共2页ACTA Academiae Medicinae Nantong

摘　　要：目的 :为了研究血小板生成素 (TPO)在慢性血小板减少性疾病中的病理变化。方法 :我们用敏感的双抗体夹心法酶联免疫吸附试验 (ELISA)检测了 40例再生障碍性贫血 (AA)和 32例特发性血小板减少性紫癜 (ITP)患者血浆 TPO的浓度。结果 :发现AA患者血浆 TPO浓度 (774± 393pg/ml)明显高于正常人 (5 5± 34pg/ml,P<0 .0 1) ,并且与血小板计数呈负相关 ;ITP患者血浆TPO水平正常或仅轻度增高 (73± 36 pg/ml) ,与正常人相比无显著性差异 (P>0 .0 5 ) ,其血浆 TPO浓度与血小板计数间也无相关性。结论 :AA和 ITP患者血浆 TPO含量不仅受到外周血血小板数量的影响同时也受骨髓巨核细胞的调节。血浆 TPO含量的检测有助于血小板减少性疾病的诊断及病理研究。Objective:To evaluate the role of thrombopoietin (TPO) in the pathobiology of chronic thrombocytopenic disease.Methods:we measured the endogenous plasma concentration of TPO in 40 patients with acquired aplastic anaemia (AA) and 32 patients with idiopathic thrombocytopenic purpura (ITP) by a sensitive sandwich enzyme-linked immunosorbent assay (ELISA). Results:The plasma TPO concentrations were significantly higher in AA patients (774±393 pg/ml) when compared to healthy controls subjects (55±34 pg/ml,P<0.01) and there was a significant negative correlation between their plasma TPO concentrations and platelet counts. In the patients with ITP, however, the TPO levels was normal or only slightly elevated (73 ±36pg/ml) and have no statistically significant difference compared to healthy controls (P>0.05). There was also no relationship between their plasma TPO levels and platelet counts. Conclusion:Our results suggest that TPO levels may be regulated not only by platelets but also megakaryocytes in AA and ITP, and measurement of TPO levels is useful for the diagnosis of thrombocytopenia and for understanding the pathophysiology of thrombocytopenia.

关 键 词：再生障碍性贫血 特发性血小板减少性紫癜 血小板生成素 酶联免疫吸附试验 

分 类 号：R558.2[医药卫生—血液循环系统疾病]