曙红B荧光猝灭法测定司帕沙星  

Determination of Sparfloxacin by Fluorescence Quenching of Eosine B

作　　者：李华岑[1] 高建华[1] 龚雪云[1] 

机构地区：[1]郑州大学化学系,郑州市450052

出　　处：《光谱实验室》2003年第5期676-680,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：河南省教委自然科学基金资助项目 (No.9815 0 0 0 7)

摘　　要：基于司帕沙星对曙红 B的荧光具有猝灭特性 ,建立一种测定微量司帕沙星的方法。在 p H=3.5的Clark- Lubs缓冲溶液中 ,曙红 B激发波长 λex=30 8nm、发射波长 λem=5 40 nm,司帕沙星的浓度在 0 .2—4 mg· L-1范围内符合比耳定律 ,相关系数为 0 .997,检出限为 0 .0 5 4mg· L-1。该方法对胶囊中的司帕沙星进行回收 ,回收率为 90 %— 10 9% ,相对标准偏差为 1.2 %— 2 .8%。猝灭机理的初步研究 ,认为主要是静态猝灭 ,求得司帕沙星与曙红 B的结合常数为 7.94× 10 4,结合比为 1.0 2。A method for the determination of sparfloxacin is developed based on the fluorescence quenching of eosine B with sparfloxacin.In pH=3.5 Clark-Lubs buffer solution,the excitation and emission wavelength were found to be 308nm and 540nm,respectively.The linear range is 0.2-4mg·L -1 for sparfloxacin with detection limit of 0.054mg·L -1.The method was applied to determine the sparfloxacin capsule with recovery of 90%-109% and relative standard deviation of 1.2%-2.8%.The quenching mechanism was considered as static quenching with the binding constant of eosine B and sparfloxacin of 7.94×10 4 and the radio of 1.02.

关 键 词：曙红B 荧光猝灭法 测定 司帕沙星 检测方法 喹诺酮类广谱抗菌药 

分 类 号：R978.1[医药卫生—药品] R917[医药卫生—药学]