利用发光酶基因监测根瘤菌重组质粒的转移性  

Monitoring the Transfer of Recombinant Plasmid pHN307 in Soybean Rhizobia by luxAB Marker Gene

作　　者：李友国[1] 周俊初[1] 

机构地区：[1]华中农业大学农业部农业微生物重点实验室,湖北武汉430070

出　　处：《微生物学杂志》2003年第6期1-4,共4页Journal of Microbiology

基　　金：国家863高技术研究发展计划课题(2001AA21401)资助

摘　　要：经三亲本杂交,比较测定了重组大豆根瘤菌HN01DNL和TA11DNL中所含重组质粒pHN307在人工滤膜和灭菌土壤杂交条件下、向华癸中生根瘤菌7653R和荧光假单胞菌Pf.X1-5的转移频率;并初步跟踪了pHN307在根盒-土壤缩影、小区试验和环境释放中向土著细菌的转移性,为考察所构建重组根瘤菌在田间应用时的安全性提供了一定的实验依据。By using the method of tri-parental matting, the transfer frequencies of recombinant plasmid pHN307 in the re-combinant soybean rhizobia HNOIDNL and TAllDNL to Mesorhizobium huakuii 7653R and Pseudomonas sp. X1-5 were determined and compared under filter and sterilized soil hybridization conditions. The transfer of pHN307 to indigenous soil bacteria was also monitored respectively in rhizobox-soil mocrocosm, field experiment and environmental releasing by luxAB marker gene. The results were much useful to evaluate the bio-safety of recombinant rhizobia constructed in a large-scale field application.

关 键 词：重组质粒 转移性 发光酶基因 大豆根瘤菌 

分 类 号：Q939.114[生物学—微生物学] Q78