体外铅暴露对发育脑海马第Ⅱ组mGluRs基因表达的影响  

Gene Expression of Metabotropic Glutamate Receptors Group Ⅱ of Embryonic Rat Hippocampus Induced by Lead Exposure

作　　者：颜崇淮[1] 徐健[1] 余晓刚 沈晓明[1] 

机构地区：[1]上海第二医科大学新华医院环境医学实验室上海市儿科医学研究所,上海200092

出　　处：《上海第二医科大学学报》2003年第B10期1-4,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金(30070665);上海市科委科技启明星计划(99QBI4008)资助项目

摘　　要：目的 研究铅暴露对发育脑海马第Ⅱ组代谢性谷氨酸受体(mGluRs)基因表达的影响。方法 在体外培养3d的原代胚鼠海马神经元中加入氯化铅暴露3周,用实时荧光定量RT-PCR方法,检测不同程度的铅暴露对胚鼠海马神经元第Ⅱ组mGluRs基因表达的影响。结果 胚鼠海马神经元铅暴露后,第Ⅱ组mGluRs基因表达较正常对照组增高,铅暴露水平不同,诱发的基因表达变异情况亦不同。结论 铅暴露引起发育脑海马第Ⅱ组mGluRs基因的上调可能是其影响学习记忆的机制之一。Objective To determine the gene expression of embryonic hippocampus metabotropic glutamate receptors group Ⅱ induced by lead exposure. Methods Primary embryonal rat hippocampus neurons were cultured for three days and these cells were exposed with lead chloride for three weeks. Real time quantitative RT - PCR was used to determine the influence of lead exposure on the gene expression of metabotropic glutamate receptors group Ⅱ. Results Metabotropic glutamate receptors group Ⅱ were up-regulated and different levels of the lead exposure resulted in different gene expression level. Conclusion Lead exposure may do harm to learning and memory through influencing the expression of metabotropic glutamate receptors group Ⅱ.

关 键 词：体外铅暴露 发育 脑海马 第Ⅱ组 MGLURS 基因表达 聚合酶链反应 代谢性谷氨酸受体 

分 类 号：R114[医药卫生—卫生毒理学]