花椒品种的DNA分子甄别  

The Molecular Authentication of Zanthexylum bungeanum Maxim. Population

作　　者：沈洁[1] 丁小余[1] 张卫明[2] 保曙琳[1] 常俊[1] 唐凤[1] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《中国野生植物资源》2003年第5期44-48,共5页Chinese Wild Plant Resources

基　　金：国家农业领域十五攻关重点项目(2 0 0 1BA50 2 B05)

摘　　要：本文对花椒不同居群的 r DNA ITS区序列片段进行了测定和分析。 r DNA ITS区序列片段有 60 3 bp、GC百分比为 60 .3 %。在所比较的花椒居群中 ,ITS区序列片段存在着 2 2个变异位点。依据花椒 r DNA ITS区的碱基差异 ,我们可以有效鉴别陕西凤县凤椒、甘肃武都大红袍。The rDNA ITS regions of populations of Zanthexylum bungeanum Maxim. in main habitant of China have been sequenced for authenticating the populations of Zanthexylum bungeanum Maxim . The rDNA ITS sequence of those populations range 603bp .The numbers of variable sites are 22.The ITS region differences between the populations of Zanthexylum bungeanum Maxim. are obvious.The difference of the rDNA ITS region sequences can be used to authenticate accurately the populations of Zanthexylum bungeanum Maxim.

关 键 词：花椒 品种 DNA分子甄别 居群差异 

分 类 号：S573.1[农业科学—作物学]