反相高效液相色谱法测定化橘红中野漆树苷的含量  

Determination of the Rhoifolin in Exocarpium Citri Grandis By RP-HPLC

作　　者：袁旭江[1] 林励[1] 李向明[2] 

机构地区：[1]广州中医药大学,广州510405 [2]化州市绿色生命有限公司,广东化州525100

出　　处：《中药新药与临床药理》2003年第6期396-398,共3页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技计划项目(编号A301020101)

摘　　要：目的 对化橘红中野漆树苷进行含量测定研究。方法 采用反相高效液相色谱法,色谱柱为MACHEREY-NAGEL 100-5 C_(18)柱(250 mm×4.6 mm,5 μm),流动相为甲醇和水-乙酸(61:4),进行梯度洗脱,流速为1.0mL/min,检测波长345 nm。结果 在0.816～13.056μg范围内,野漆树苷与色谱峰面积呈良好的线性关系,r=0.9995,平均回收率为101.064％,RSD=3.10％(n=5)。结论 建立了测定化橘红中野漆树苷含量的HPLC法,该方法简便准确,重现性好;毛橘红和光橘红中野漆树苷含量有显著差异。Objective To establish a method for the determination of Rhoifolin content in Exocarpium citri grandis (ECG). Methods The RP-HPLC method was operated on MACHEREY-NAGEL 100-5 C_(18), with methanol and wateracetic acid (61: 4) as mobile phase, gradient elution, flow rate at 1.0 mL/min, and detection wavelength at 345nm. Results In the range from 0.816 μg to 13. 056μg, Rhoifolin is in a good linearity with chromatographic peaks area, r=0.9995, the average recovery ratio is 101.064%, RSD=3.10%(n=5). The content of Rhoifolin in Tomentulosus Exocarpium Citri Grandis(TECG) ranged from 1.33% to 0.177% and that in Glabrous Exocarpium Citri Grandis (GECG) is less than 0.17%. Conclusions RP-HPLC method is with accuracy and a good reproducibility for the determination of the Rhoifolin content in ECG; the difference of Rhoifolin content in TECG and GECG is significant.

关 键 词：化橘红/化学 野漆树苷/分析 高压液相色谱法 

分 类 号：R284.1[医药卫生—中药学]