补肾益智方对卵巢切除大鼠海马长时程增强的影响  

Effect of BushenYizhi Prescription on Long-Term Potentiation in Hippocampus of Ovariectomized Rats

作　　者：周杰明[1] 赖世隆[2] 饶燕[2] 胡镜清[3] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]广州中医药大学DME中心,广州510405 [3]中国中医研究院临床评价中心,北京100700

出　　处：《中药新药与临床药理》2003年第6期369-371,共3页Traditional Chinese Drug Research and Clinical Pharmacology

摘　　要：目的 观察补肾益智方对卵巢切除大鼠海马长时程增强(LTP)的影响。方法 3月龄SD雌性大鼠,随机分为假手术组、模型组、补肾益智方低剂量组、补肾益智方高剂量组、雌激素对照组,模型组行双侧卵巢切除术,补肾益智方高、低剂量组、雌激素对照组分别在卵巢切除术后予补肾益智方灌胃和结合雌激素皮下注射。运用电生理学检测方法,观察大鼠长期雌激素剥夺对大鼠海马长时程增强的影响以及中药补肾益智方的干预作用。结果与结论卵巢切除大鼠海马齿状回产生LTP的幅度明显低于卵巢保留组,并且维持的时间也较短;雌激素替代和补肾益智方高剂量组的LTP增幅和维持时间与卵巢保留组相比均无明显降低。说明二者使卵巢切除大鼠的突触易化机制得以保护和维持。Objective To observe the effect of Bushen Yizhi Prescription(BYP) on long-term potentiation (LTP) in hippocampus of ovariectomized (OVX) rats. Methods Three-month-old female SD rats were randomized into mimic operation group, model group, low dosage BYP group, high dosage BYP group and estrogen control group. Ovariectomy was operated in the three latter groups. After operation, BYP group was treated with gastric infusion of BYP and estrogen group with subcutaneous injection of estrogen. The changes of LTP of dentate gyms in hippocampus of OVX rats were observed by electrophysiological method and the effect of BYP on LTP was also evaluated. Result & Conclusion The increased amplitude of population spike of LTP was significantly low and maintained a short time in model group as compared with that in mimic operation group. There were insignificant difference between the high-dosage BYP group and estrogen control group. It is indicated that synaptic transmission in hippocampus can be protected and sustained by BYP and the estrogen replacement.

关 键 词：补肾益智方/药理学 长时程增强 电生理学 

分 类 号：R285.5[医药卫生—中药学] R338.64[医药卫生—中医学]