Surfactant-coated Candida rugosa Lipase as Catalyst for Hydrolysis of Olive Oil in Solvent-Free Two-Phase System  

作　　者：宋宝东 丁辉 吴金川 Hayashi Y. Talukder MMR 王世昌 

机构地区：[1]Chemical Engineering Research Center, School of Chemical Engineering and Technology Tianjin University Tianjin 300072,China [2]Department of Chemistry & Chemical Engineering, Faculty of Engineering, Kanazawa University 2-40-20 Kodatsuno, Kanazawa 920-8667, Japan [3]Chemical Engineering Research Center, School of Chemical Engineering and Technology Tianjin University Tianjin 300072, China

出　　处：《Chinese Journal of Chemical Engineering》2003年第5期601-603,共3页中国化学工程学报（英文版）

基　　金：National Natural Science Foundation of China(No.29876031)

摘　　要：The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases,the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.The surfactant-coated Candida rugosa lipase was used as catalyst for hydrolysis of olive oil in two-phase system consisting of olive oil and phosphate buffer without organic solvent. For both the coated and native lipases, the optimal buffer/oil volume ratio of 1.0, aqueous pH 6.8 and reaction temperature 30℃ were determined. The maximum activity of the coated lipase was ca 1.3 times than that of the native lipase. The half-life of the coated lipase in olive oil and the native lipase in phosphate buffer was ca 9 h and 12 h, and the final residual activity was 27% and 20% of their initial values, respectively. The final substrate conversion by the coated lipase was ca 20% higher than that of the native lipase.

关 键 词：Candida rugosa HYDROLYSIS LIPASE olive oil solvent-free system SURFACTANT 

分 类 号：O621[理学—有机化学]