益气化瘀方对大鼠腰神经根受压后神经肌肉终板再生修复的作用  

Effect of Yiqi Huayu Recipe on regeneration process after L_5 nerve root compression in rats

作　　者：周重建[1] 王拥军[1] 施杞[1] 侯宝兴[1] 刘梅[1] 

机构地区：[1]上海中医药大学脊柱病研究所

出　　处：《中西医结合学报》2003年第4期277-280,共4页Journal of Chinese Integrative Medicine

基　　金：上海市自然科学基金资助项目 (No .0 2ZB14 0 98)

摘　　要：目的　探讨大鼠L5神经根受压后 ,在神经再生修复过程中 ,益气化瘀方对神经肌肉接头部神经元的作用。方法　 4 8只大鼠随机分为 10、2 0、30、6 0d对照组和益气化瘀方组。制作大鼠L5神经根受压模型 ,益气化瘀方组给予益气化瘀方灌胃 ,对照组给予等量生理盐水。造模后 10、2 0、30、6 0d取大鼠比目鱼肌 ,采用多克隆蛋白基因产物 9.5作为神经元标记 ,应用免疫组织化学法结合激光共聚焦扫描显微技术 ,观察神经肌肉接头部末梢神经再生修复的过程。α环蛇毒素荧光结合剂显示运动终板 ,NIH图像分析技术测定末梢神经与运动终板的重叠面积。结果　肌肉失神经支配后 ,益气化瘀方组的末梢神经在神经肌肉接头部的聚集、出芽及延伸均显著地早于对照组。在神经再生修复期间 ,益气化瘀方组的末梢神经与运动终板的重叠速度、范围及神经肌肉接头的再构筑 ,亦显著地优于对照组。结论　益气化瘀方能促进神经元的增生 ,增强其再生能力 ,加快神经肌肉接头的重建 。Objective To study the effect of Yiqi Huayu Recipe on neurons in neuromuscular junction after L 5 nerve root compression in rats. Methods By using immunohistochemistry,confocal laser scanning techniques and protein gene product 9.5 as a neuronal marker,the motor endplates were visualized with fluorescein-conjugated α-BTX. The effect of Yiqi Huayu Recipe on nerve terminals of neuromuscular junction at 10,20,30,60 days after L 5 nerve root compression was observed in rat soleus muscles during regeneration. The overlap areas of terminals and endplates were measured with NIH image technique. Results As for the aggregates, sprouts, extension of nerve terminal during regeneration, the connecting speed and range of nerve terminal with endplates, as well as the reformation of neuromuscular junctions during regeneration, Yiqi Huayu Recipe group was better than that of the control group. Conclusion Yiqi Huayu Recipe can promote the hyperplasia of neurons and enhance the regeneration function. It also can accelerate the neuromuscular junction reformation and shorten the process of regeneration.

关 键 词：益气化瘀方 大鼠 腰神经根 神经肌肉 中草药 脊神经根 免疫组织 

分 类 号：R285.5[医药卫生—中药学]