CO_2和O_3浓度倍增及其交互作用对大豆叶绿体超微结构的影响  

Effect of doubled CO_2 and O_3 concentration and their interactions on ultrastructure of soybean chloroplast

作　　者：赵天宏[1] 史奕[1] 黄国宏[1] 

机构地区：[1]中国科学院沈阳应用生态研究所

出　　处：《应用生态学报》2003年第12期2229-2232,共4页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目 (4 0 2 71111);中国科学院沈阳应用生态研究所领域前沿创新资助项目 (SCXMS0 3 0 2 )

摘　　要：应用透射电镜观察了模拟大气CO2 和O3 浓度倍增及其交互作用 (开顶箱法 )对大豆叶肉细胞叶绿体超微结构的影响 .结果表明 ,CO2 浓度倍增促进了大豆叶绿体的发育 ,内含淀粉粒积累明显增多、体积增大 ;叶绿体被膜保持完好 ;叶绿体基粒片层排列整齐 .而O3 浓度倍增抑制了叶绿体内淀粉粒的累积 ,并导致叶绿体被膜破碎 ,片层解体 ,严重地破坏了叶绿体的结构和功能 .CO2 和O3 浓度倍增的交互作用对叶绿体超微结构有不同程度的破坏 ,但二者浓度呈梯度增加对叶绿体的损害作用要大于二者浓度持续倍增对叶绿体的影响 ,进一步表明CO2 正效应对O3The effect of doubled CO 2 and O3 concentration on the ultrastructure of chloroplast in leaf cells of soybean was tested by open top chamber method,and examined under transmission electron microscope. The results showed that under doubled CO 2 concentration,the amount and volume of starch grains in chloroplast increased,but the chloroplast membrane and the lamellar structure still kept integrated. The accumulation of starch grains was restrained,and the chloroplast membrane and the lamellar structure were disorganized under doubled O 3 concentration. The ultrastructure of chloroplast was destroyed in different degrees under the interaction of doubled CO 2 and O 3 concentration. The damage to chloroplast arose from the abrupt doubling of CO 2 and O 3 was more severe than that from gradual increase of CO 2 and O 3 concentration. The negative effect of O 3 was partly compensated by the positive effect of doubled CO 2.

关 键 词：CO2 O3 交互作用 叶绿体超微结构 大豆 

分 类 号：S565.1[农业科学—作物学]