水稻化感材料控制稗草的基因定位研究  

Genes mapping on rice allelopathy against barnyardgrass

作　　者：徐正浩[1] 何勇[1] 崔绍荣[1] 赵明[2] 张旭[2] 李迪[3] 

机构地区：[1]浙江大学生物系统工程及食品科学学院 [2]中国农业大学作物学院,北京100094 [3]中国水稻研究所,杭州310006

出　　处：《应用生态学报》2003年第12期2258-2260,共3页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目 (3 0 170 62 0 );国家"十五"科技攻关资助项目 (2 0 0 0 1BA5 0 9B0 7)

摘　　要：利用中 15 6 /谷梅 2号建立的重组自交系 (RILs)所构建的包括 16 8个DNA标记 ,全长为 14 4 7.9cM ,基本覆盖水稻基因组 12条染色体的连锁图 ,用差时播种共培法的改进方法对 134个该群体的株系及其亲本对无芒稗进行了化感作用评价 .用无芒稗的植株干重作为表型定位水稻化感控制稗草的基因 .用QTLMapper 1.0 1b软件进行区间作图 ,检测到 1个与化感作用有关的主效应QTL .该QTL位于第 7条染色体上 ,解释了 32 .30 %的表型变化 ;检测到 6对上位QTL ,解释了 4 7.83%的无芒稗干重抑制的变化 ,主效应和上位效应QTL共解释了 80 .By using the relay seeding technique with slight modification,a population of 134 recombinant inbred lines (RILs )derived from a cross between indica rice ( Oryza sativa L.) cultivar Zhong 156× Gumei 2 and contained 168 DNA markers covering all 12 chromosomes with 1447.9 cM spans was employed to evaluate the rice allelopathic effect on barnyardgrass [ Echinochloa crus galli (l.) Beauv.]. The phenotyping values of the biomass of barnyardgrass infested with rice materials were employed to map the genes of allelopathic activity on barnyardgrass. One main effect of QTL on 7 chromosomes was identified,which explained 32.30% of the phenotypic variation. Six pairs of digenic epistatic loci were also detected,which collectively explained 47.83% of the phenotypic variation. The main QTL and epistatic loci totally explained 80.13% of the phenotypic variation of allelopathic activity.

关 键 词：水稻 差时播种共培法 化感作用 无芒稗 QTL 

分 类 号：S451[农业科学—植物保护]