动物生物标志物在土壤污染生态学研究中的应用  

Application of terrestrial invertebrates biomarkers in soil pollution ecology study

作　　者：李培军[1] 熊先哲[1] 杨桂芬[1] 刘宛[1] 许华夏[1] 台培东[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《应用生态学报》2003年第12期2347-2350,共4页Chinese Journal of Applied Ecology

基　　金：国家自然科学基金项目 (2 0 2 770 40 );中国科学院知识创新工程资助项目 (KZCXZ SW 416;SCXZD0 10 3 )

摘　　要：应用陆栖无脊椎动物的生物标志物对土壤生态系统中污染物的暴露和效应进行评价日益受到重视 ,并取得了显著的研究进展 .文中介绍了溶酶体、胁迫蛋白和金属硫蛋白 (MTs) 3种主要生物标志物 .体腔细胞内溶酶体膜稳定性用中性红保持时间 (NRR)进行检测 ;胁迫蛋白类多采其中的Hsp70和Hsp6 0 ;金属硫蛋白不同同分异构体的定量分析可用于反映不同的金属污染胁迫 .对 3种生物标志物机理、特性、检测实例以及在污染土壤生态毒理诊断中的应用前景进行了评述 .Biomarkers in terrestrial invertebrates play an important role in estimating either exposure or resultant effects of pollutants in soil ecosystem,which has received increasing attention and made significant progress. The present paper has drawn three important biomarkers,i.e.,lysosomes,stress protein and mettllothioneins (MTs). The lysosomal membrane stability of coelomocytes was assayed as neutral red retention time (NRR time) resulting from toxicant stress. Hsp70 and Hsp60 were commonly used in the stress protein families. The quantification methods of different isofoms of the mettllothioneins could specifically indicate different metal contamination. The rationale,characteristics,and relevant exemplary case of assaying the biomarkers and their promising application to ecotoxicological diagnosing in soil contamination were discussed.

关 键 词：生物标志物 溶酶体 胁迫蛋白 金属硫蛋白 

分 类 号：X53[环境科学与工程—环境工程]