高效液相色谱法测定酮咯酸氨丁三醇注射液的含量  

Determination of the content of ketorolac tromethamine in injection by HPLC

作　　者：阳国平[1] 贺彬[1] 刘世坤[1] 吴翠芳[1] 

机构地区：[1]中南大学湘雅三医院,湖南长沙410013

出　　处：《中国医院药学杂志》2003年第12期733-734,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立一种用高效液相色谱法测定酮咯酸氨丁三醇注射液含量的方法。方法 :采用HypersilODS2C185 μm柱 ,甲醇 水 冰醋酸 (5 5∶4 4∶1)为流动相 ,流速约为 1.2mL·min-1,检测波长为 2 4 9nm。结果 :该方法酮咯酸氨丁三醇含量线性范围为 0 .0 96～ 0 .336g·L-1,r =0 .9999,回收率为 99.38% ,RSD为 0 .4 2 % (n =6 )。结论 :该方法简便、准确 ,能满足制剂质量标准的要求。OBJECTIVE To establish an HPLC method for the determination of ketorolac tromethamine in injection.METHODS The chromatographic system consisted of Hypersil ODS2 C_ 18 column and mobile phase of methanol-water-glacial acetic acid(55∶44∶1)with a detection wavelength at 249 nm.RESULTS The linear range of ketorolac tromethamine was 0.096 ～ 0.336 g·L -1 , r = 0.9999 ;the average recovery and relative standard deviation were 99.34% , 0.35% .CONCLUSIONS This method is simple and accurate for determination of ketorolac tromethamine in injection,can satisfy the request of quality standard of preparation.

关 键 词：酮咯酸氨丁三醇 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]