高效液相色谱法测定更昔洛韦滴眼液的含量  

Determination of ganciclovir eye drops by HPLC

作　　者：唐细兰[1] 陈雷宇[1] 吴伟[1] 叶成添[1] 

机构地区：[1]中山大学中山眼科中心,广东广州510060

出　　处：《中国医院药学杂志》2003年第12期740-741,共2页Chinese Journal of Hospital Pharmacy

摘　　要：目的 :建立测定更昔洛韦滴眼液含量的高效液相色谱法。方法 :以HypersilC18为色谱柱 ,甲醇 水 (10∶90 )为流动相 ,检测波长 2 5 2nm。结果 :更昔洛韦在 2 .5～ 4 0mg·L-1浓度范围内线性关系良好 (r =0 .9998) ,平均回收率为 10 0 .7% ,RSD为0 .33%。结论 :本方法简便、快速、灵敏 ,可作为该滴眼液的质量控制。OBJECTIVE To establish an HPLC method for determining the content of ganciclovir eye drops.METHODS Analytical column was Hypersil C_ 18 ,the mobile phase was methanol-water(10∶90). The UV detection wavelength was 254 nm.RESULTS There was a good linear relationship within the concentration range of 2.5 ～40 mg·L -1 in ganciclovir eye drops( r = 0.9998 ).The average recovery of ganciclovir was 100.7% ,RSD was 0.33% .CONCLUSIONS This method is simple,rapid and accurate.

关 键 词：更昔洛韦 高效液相色谱法 含量 

分 类 号：R927.2[医药卫生—药学]