六味地黄方药物血清对血管内皮细胞胞内Ca^(2+)的影响  

Influence on Ca^(2+) in vascular endothelial cell treated with drug serum of Liuweidihuang Formula (六味地黄方)

作　　者：张旭[1] 卞慧敏[1] 张超英[2] 

机构地区：[1]南京中医药大学,南京210029 [2]南京师范大学生命科学院,南京210024

出　　处：《中药药理与临床》2003年第5期1-2,共2页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家中医药管理局资助课题 (2 0 0 0 J Q 0 8) ;江苏省中医药局课题 (H 0 0 4 );江苏省教育厅自然科学基金 (0 2KJB360 0 0 5)

摘　　要：目的 :探讨六味地黄方药物血清保护血管内皮细胞的分子机制。方法 :取人脐静脉内皮细胞 (HUVEC) ,用脂多糖(LPS)造成凋亡细胞模型 ,通过激光共聚焦显微镜观察六味地黄方药物血清对胞内Ca2 + 的影响。结果 :六味地黄方药物血清可明显缓解其所致的钙超载。结论 :六味地黄方药物血清有抗HUVEC凋亡作用 ,其防治血栓性疾病的机理可能与其缓解细胞钙超载有关。Objective: To explore the molecular mechanism of Liuweidihuang Formula (LWDHF) against vascular endothelial cell (VEC) apoptosis. Method: The apoptosis model of human umbilical vascular endothelial cell (HUVEC) was induced by lipopolysaccharide (LPS), the influence of LWDHF on Ca 2+ was detected by flow cytometry and laser confocal microscope. Result: the LWDHF serum released the overloading of Ca 2+. Conclusion: The mechanism of LWDHF against HUVEC apoptosis and thrombus diseases is related with its remission of Ca 2+ overloading.

关 键 词：六味地黄方 血清 血管内皮细胞胞 钙离子 分子机制 血栓性疾病 

分 类 号：R285[医药卫生—中药学]