Triton X-100快速提取甲醛固定、石蜡包埋组织内DNA的方法  

A rapid method of DNA extra ction from formalin-fixed paraffin-embedded tissues by using Triton X-100

作　　者：贾怡[1] 周斌[1] 吕梅励[1] 梁伟波[1] 胡羽[1] 张林[1] 

机构地区：[1]四川大学基础医学与法医学院教育部人类疾病生物治疗重点实验室,成都610041

出　　处：《刑事技术》2003年第6期6-8,23,共4页Forensic Science and Technology

基　　金：国家自然科学基金(30171033);教育部科学技术研究重点项目(01143);教育部跨世纪优秀人才培养计划基金(2001-29)

摘　　要：目的建立一种快速提取甲醛固定、石蜡包埋组织内DNA的方法。方法利用TritonX-100一步提取甲醛固定、石蜡包埋组织内DNA,并具体研究了不同浓度TritonX-100对提取后DNA-STR分型效果的影响。结果成功地一步提取出甲醛固定、石蜡包埋组织内DNA,浓度为1%的TritonX-100提取效果较好。结论利用TritonX-100提取甲醛固定、石蜡包埋组织内的DNA,为快速提取DNA提供了有效的途径,是法科学领域中一种值得推荐的方法。Objective Introducing a rapid method of DNA extract ion from formalin-fixed paraffin-embedded tissues.Methods The DNA of tissues from formalin-fixed paraffin-embedded are extracted by using Triton X-100 and the effect of DNA extract quantity in different concentration of Triton X-100solutions is analyzed.Result DNA are extracted successfully from formalin-fixed paraffin-embedded tissues and we find that a better DNA-STR typing re sult can be got by using1%Triton X-100solution.Conclusion It is determine d to be a valid method for rapidly extracting DNA from formalin-fixed paraffin-embedded tissues using Triton X-100and should be recommended.

关 键 词：甲醛固定 石蜡包埋组织 DNA鉴定 法医学 案件侦查 TRITONX-100 非离子表面活性剂 

分 类 号：D919.2[医药卫生—法医学]