RG50864对PDGF诱导的肺动脉平滑肌细胞增殖的影响  

Effect of RG50864 on Pulmonary Artery Smooth Muscle Cell Proliferation of Platelet Derived Growth Factor Induced

作　　者：王昌明[1] 黄慧[1] 曾锦荣[1] 莫碧文[1] 王绩英[1] 白晶[1] 

机构地区：[1]桂林医学院附属医院呼吸疾病研究室,广西桂林541001

出　　处：《华夏医学》2003年第6期771-772,共2页Acta Medicinae Sinica

基　　金：广西壮族自治区青年科学基金资助 (桂科青 0 0 0 70 2 2 )

摘　　要：目的 :研究酪氨酸蛋白激酶抑制剂 RG5 0 86 4对 PDGF诱导的肺动脉平滑肌细胞增殖的影响。方法 :用原代培养的小牛肺动脉平滑肌细胞 ,采用细胞计数法、MTT比色法及 Giemsa染色法对 PDGF诱导的肺动脉平滑肌细胞进行分析。结果 :与对照组相比 ,RG5 0 86 4处理组能在 2 4 h、4 8h、72 h显著抑制 PDGF诱导的肺动脉平滑肌细胞增殖 ,且呈剂量依赖性。结论 :RG5 0 86 4可显著抑制Objective:To explore the effect of tyrosine protein kinase(TPK) inhibitor RG50864 on Pulmonary artery smooth muscle cell(PASMC) proliferation of platelet derived growth factor(PDGF) induced.Methods:By primary culture of calf PASMC,the effect of PDGF induced PASMC proliferation was analysed using MTT colorimetry,Giemsa staining and cell count.Results:As compared with control group,PDGF induced PASMC proliferation was significantly inhibited by RG50864 groups in a dose dependent manner.Conclusion:RG50864 could block PDGF induced PASMC proliferations.

关 键 词：酪氨酸蛋白激酶 血小板衍生生长因子 肺动脉平滑肌细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学] R332.2[医药卫生—基础医学]