Patterns of mouse reticulon 3 mRNA and protein expression in the mouse central nervous system  

作　　者：LIUJin HUANGXiaowei PENGXiaozhong YANGHongbo YINBin TANXinyu FANMing FANWenhong LIUBingyan QIANGBoqin YUANJiangang 

机构地区：[1]NationalLaboratoryofMedicalMolecularBiology,InstituteofBasicMedicalSciences,PekingUnionMedicalCollege&ChineseAcademyofMcdicalSciences,NationalHumanGenomeCenter,Beijing100005,China [2]DepartmentofNeurobiology,InstituteofBasicMedicalScience,Beijing100850,China [3]PekingUnionMedicineCollegeHospital,Beijing100005,ChinaCorrespondenceshouldbeaddressedtoYuanJiangang

出　　处：《Chinese Science Bulletin》2003年第19期2044-2049,共6页

基　　金：This work was supprted by the National Key Basic Rescarch Project(Grant Nos.G1998051002 and 001cb510206);the National High Technology Research and Development Program(Grant Nos.2001AA221041 and 2002BA711A01-03);the National Natural Sciences Foundation of China(Grand No.39830070).

摘　　要：Reticulons (RTN) are endoplasmic reticulum- associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are still elusive. In the present study, mouse reticulon 3 (mRTN3) is cloned and its expression pattern in a variety of tissues is investigated. Three alternatively spliced transcripts of 1.8, 2.8 and 4.2 kb are revealed by Northern blotting hybridization. The 1.8 and 2.8 kb transcripts are expressed in many tissues. The 2.8 kb transcript has a high level in brain and the 4.2 kb transcript is only found in brain. In situ hybridization and immunohistochemical analysis indicated its high expression in non-glial cells in some particular region of mouse central nervous system, such as hippocampus, sub-thalamus nucleus, thalamus nucleus and cerebrum cortex.Reticulons (RTN) are endoplasmic reticulum- associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are still elusive. In the present study, mouse reticulon 3 (mRTN3) is cloned and its expression pattern in a variety of tissues is investigated. Three alternatively spliced transcripts of 1.8, 2.8 and 4.2 kb are revealed by Northern blotting hybridization. The 1.8 and 2.8 kb transcripts are expressed in many tissues. The 2.8 kb transcript has a high level in brain and the 4.2 kb transcript is only found in brain. In situ hybridization and immunohistochemical analysis indicated its high expression in non-glial cells in some particular region of mouse central nervous system, such as hippocampus, sub-thalamus nucleus, thalamus nucleus and cerebrum cortex.

关 键 词：MRNA 蛋白质 中枢神经系统 鼠 模型 RTN 原位杂交 免疫组织化学反应 神经内分泌特效蛋白质 

分 类 号：R392[医药卫生—免疫学]