第二代杂交捕获法和聚合酶链反应检测人乳头瘤病毒的比较  

Comparison of Hybrid Capture I (HC I ) Test and Polymerase Chain Reaction Foe Detection of Human Papillomavirus

作　　者：薛耀华[1] 唐红平 汪凡军[1] 詹永玮 曾征宇[1] 朱庆义[1] 

机构地区：[1]广州金域医学检验中心,广东广州510182 [2]广州民航医院,广东广州510405

出　　处：《实用医技杂志》2003年第11期1215-1216,共2页Journal of Practical Medical Techniques

摘　　要：目的:比较两种常用的人乳头瘤病毒(HPV)的检测方法。方法:第二代杂交捕获法(HCⅡ)和聚合酶链反应(PCR)检测妇女宫颈分泌物中的高危型HPV。结果:103例宫颈病变患者中,PCR和HCⅡ检测HPV的阳性率分别为41.7％和39.9％。两种检验方法具有相关性(P<0.05)。结论:PCR和HCⅡ具有较好的一致性。Objective Two commonly used tests for human papillomavirus detection were compared. Methods Hybrid capture Ⅱ (HC Ⅱ ) test and polymerase chain reaction (PCR) were used for detection of high-risk HPV from cervical scrapes of 103 women. Results The overall HPV DNA positivity for known type was 41. 7 % by PCR compared to 39. 9 % by HC Ⅱ. There is significant relationship between HC Ⅱ and PCR (P<0. 05). Conclusion The results show good agreement between HC Ⅱ and PCR assay for HPV.

关 键 词：第二代杂交捕获法 聚合酶链反应 人乳头瘤病毒 宫颈分泌物 宫颈癌 

分 类 号：R446.5[医药卫生—诊断学]