地塞米松对大鼠星形胶质细胞诱导型一氧化氮合酶活性的影响  

Effect of Dexamethasone on Inducible Nitric Oxide Synthase Activity in Rat Astrocytes

作　　者：高峰[1] 水泉祥[1] 吕娟芬[1] 周柏林[1] 夏哲智[1] 

机构地区：[1]浙江医科大学附属儿童医院内科

出　　处：《浙江大学学报（医学版）》1999年第1期16-18,共3页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省教委资助

摘　　要：目的：探讨地塞米松对内毒素诱导的大鼠星形胶质细胞中诱导型一氧化氮合酶（ｉＮＯＳ）活性的影响。方法：应用Ｇｒｉｅｓｓ法，动态测定内毒素（ＬＰＳ）诱导体外培养的大鼠星形胶质细胞ｉＮＯＳ活性的变化以及地塞米松（ＤＥＸ）对其的影响。结果：细菌ＬＰＳ能够刺激星形胶质细胞ｉＮＯＳ活性的表达，且与ＬＰＳ剂量和作用时间有关，ＤＥＸ对星形胶质细胞ｉＮＯＳ活性表达具有负调节作用，而且与加入ＤＥＸ的间隔时间有关。结论：星形胶质细胞ｉＮＯＳ可能参与中枢神经系统细菌感染的病理过程，而ＤＥＸ能抑制这种损害作用。Objective:To examine the effect of dexamethasone on the inducible nitric oxide synthase(iNOS) activity induced by endotoxin (LPS) in cultured rat astrocytes.Methods:INOS activity in normal astrocytes,LPS induced astrocytes,and dexamethasone treated LPS induced astrocytes was detected by Griess methods.Results:INOS activity was increased by endotoxin inducement in a dose and time dependent manner.Dexamethasone inhibited iNOS activity in astrocytes induced by endotoxin,and this inhibition was associated with the delay of dexamethasone addition after LPS stimulation.Conclusion:It was suggested that the iNOS of astrocytes might have a part in the pathology of bacterial infection in central nervous system,and it's role could be decreased by DEX.

关 键 词：地塞米松 大鼠 星形胶质细胞 诱导型一氧化氮合酶活性 内毒素 药理学 

分 类 号：R977.11[医药卫生—药品] R965[医药卫生—药学]