CTLA4Ig诱导免疫耐受的体外研究  

Experimental study of immune tolerance induced by CTLA4Ig in vitro

作　　者：姜庆[1] 吴军[1] 易绍萱[1] 陈希炜[1] 罗高兴[1] 贺伟峰[1] 

机构地区：[1]第三军医大学附属西南医院全军烧伤研究所,重庆市器官移植基础研究所,创伤烧伤复合伤国家重点实验室,重庆400038

出　　处：《第三军医大学学报》2003年第21期1896-1898,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金资助重大项目(39993430-2);国家自然科学基金资助面上项目(39970756);国家科技部重点项目(96-920-20-10);全军医药重点项目(98Z081)~~

摘　　要：目的 观察CTLA4Ig在诱导人外周血T细胞免疫耐受中的作用。方法以结核菌素刺激人外周血淋巴细胞,观察不同剂量CTLA4Ig对外周血淋巴细胞增殖反应的影响;观察CTLA4Ig和CsA(环孢霉素A)对再次结核菌素刺激和非相关第三者APC细胞刺激淋巴细胞增殖反应的影响,IL-2的变化,以及外源性IL-2对免疫耐受诱导的影响。结果CT-LA4Ig抑制淋巴细胞增殖呈剂量依赖关系,20μg/ml时抑制作用最强;CTLA4Ig能诱导免疫耐受并与CsA有协同作用;外源性IL-2能抑制免疫耐受的诱导。结论CTLA4Ig能抑制淋巴细胞的增殖和IL-2的分泌释放,CTLA4Ig和CsA联合应用能更彻底抑制淋巴细胞增殖和IL-2的分泌释放,并诱导免疫耐受;外源性IL-2能逆转免疫耐受。Objective To study the roles of cytologic T lymphocyte-associated antigen 4 immunoglobulin ( CTLA4Ig) in the induction of human T cell immune tolerance. Methods The effects of different doses of CTLA4Ig on the human T cell proliferation induced by tuberculin as well as those of CTLA4Ig and cyclosporin A (CsA) on the human lymphocyte proliferation re-stimulated by tuberculin or by the third party antigen-presenting cells (APC) were observed. The changes of IL-2 and effect of IL-2 on the immune tolerance were also observed. Results Tuberculin-induced T cell proliferation was inhibited by CTLA4Ig in a dose-dependent manner. The inhibitory effect was the strongest when the dose of CTLA4Ig was 20 fig/ml. CTLA4Ig could induce the immune tolerance and had a cooperative effect with CsA, but IL-2 could inhibit the immune tolerance. Conclusion CTLA4Ig can inhibit T cell proliferation and IL-2 excretion. Combination of CTLA4Ig and CsA can completely inhibit T cell proliferation and IL-2 excretion but can induce immune tolerance. The immune tolerance can be reversed by IL-2.

关 键 词：CTLA4IG T细胞 免疫耐受 细胞增殖 

分 类 号：R392.11[医药卫生—免疫学] R392.12[医药卫生—基础医学]