苹果煤污病与蝇粪病病原菌分离技术  

Isolation of sooty blotch and flyspeck fungi from apple surface by picking up the thalli

作　　者：孙广宇[1] 张荣[1] 章柱[1] 张苗[1] 

机构地区：[1]西北农林科技大学植保学院,杨凌712100

出　　处：《植物病理学报》2003年第5期479-480,共2页Acta Phytopathologica Sinica

摘　　要：苹果煤污病和蝇粪病的病原菌生长在果实的表面 ,由于分离过程中易于受到其它真菌和细菌的污染使得按常规方法分离相当困难。本研究使用直接挑取菌体分离技术进行了尝试 ,分离成功率高。为了保证每个样品至少长出 2～ 3个纯菌落 ,每次分离应该有 15个重复。The contaminations make it difficult to isolate sooty blotch and flyspeck fungi from apple surface. In this research we used the technique to pick up the thallus directly from apple surface under a stereomicroscope. For each sample, 10-15 slant repeats were needed to insure at least two to three isolations could grow up. Compared with common methods repor- ted previously, this technique is of high efficiency and less contamination rate.

关 键 词：苹果 煤污病 蝇粪病 病原菌 分离技术 果实 菌落 生长速度 形态 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治]