大鼠发育过程中视网膜神经节细胞的膜学特性  

Membrane properties of rat retinal ganglion cells during postnatal development

作　　者：王宗华[1] 阴正勤[1] 王仕军[1] 陈珊[1] 

机构地区：[1]第三军医大学附属西南医院眼科,重庆400038

出　　处：《第三军医大学学报》2003年第21期1936-1939,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金资助项目(30270457)~~

摘　　要：目的研究出生后不同发育阶段Wistar大鼠视网膜神经节细胞(RGCs)的膜学特性,包括被动膜学特性和去极化脉冲诱发的动作电位(Action potential,AP),并分析其与年龄的关系.方法应用视网膜切片膜片钳全细胞记录技术,获得7～30 d Wistar大鼠视网膜神经节细胞的膜片钳全细胞记录.结果①记录了112个视网膜神经节细胞;②随着发育,RGCs的膜学特性发生显著变化,兴奋性增加,睁眼前组与睁眼后组间有显著性差异;③去极化脉冲诱发其产生的动作电位有3种形式:单峰型、瞬变型、持续型.随着视觉发育,单峰型逐渐减少,持续型逐渐增加,细胞成熟时未见到单峰型,只见到瞬变型和持续型.随着发育,RGCs的电生理特性发生显著变化.结论①睁眼后形觉刺激促进RGCs膜学特性的成熟;②随着发育,RGCs的AP类型发生显著变化,单峰型是RGCs发育过程中的一种前期状态,最终分化为瞬变型和持续型RGCs.Objective To study the membrane properties of rat retinal ganglion cells during postnatal development, including the passive membrane properties and the action potentials evoked by depolarizing current injections and the relationship between the membrane properties and ages. Methods Whole cell patch clamp recordings of the retinal ganglion cells (RGCs) in retinal slices of postnatal rats (age ranging from postnatal days 7 to 30) were performed. Results (1) A total of 112 RGCs were recorded from postnatal rats. (2) The electrophysiological properties of RGCs changed significantly during development. The excitability of RGCs increased in an age-dependent manner. There was significant difference between before-eye-opening group(P7-13d) and after-eye-opening group(P14-30d) . (3) Three different discharge patterns of RGCs in response to sustained depolarizing current pulses were recorded: single-spike firing, transient firing and sustained firing. During development, retinal ganglion cells of rats exhibited pronounced changes in the discharge patterns(P <0.01) evoked by depolarizing current injections, and the electrophysiological properties of rat retinal ganglion cells changed significantly( P < 0.05). Conclusion (1) After eye opening, visual stimulation of patterns can facilitate the maturity of the membrane properties of RGCs. (2) During development, there is a clear age-related shift in the distribution of the different response patterns. The single-spike firing, a prophase state of RGCs during postnatal development, may finally change into two different types: transient firing and sustained firing.

关 键 词：神经节细胞 全细胞记录 发育 膜学特性 电生理学 视网膜 

分 类 号：R338.8[医药卫生—人体生理学] R339.41[医药卫生—基础医学]