张家口市SARS患者、疑似病例、留观及密切接触医护人员血清中IgG、IgM抗体测定  

Measurement of anti-SARS IgG and IgM Antibody in the Serum of the Patients, Suspected Person, Observed Persons, and Physicians and Nurses Closely Associated with SARS Patients in Zhangjiakou City

作　　者：牛发良[1] 张志华[1] 邹玉安[1] 舒立莎[1] 苏峰[1] 蒋贵昱[1] 崔志强[1] 冯琳琳[1] 侯亚利[1] 尹亮[1] 李艳萍[1] 薛士杰[1] 张有成[1] 

机构地区：[1]张家口医学院第一附属医院检验科,075000

出　　处：《张家口医学院学报》2003年第6期7-9,共3页

摘　　要：目的 :为临床诊断及排除严重急性呼吸综合征 (SARS)提供依据和对留观及密切接触医护人员有无感染作初步调查。方法 :用酶联免疫吸附测定 (ELISA) 5 3例临床诊断病例、2 9例疑似病例、6 3例留观及 114例密切接触医护人员的血清中SARS冠状病毒IgG、IgM抗体。结果 :SARS临床诊断病例IgG抗体阳性 2 1例 ,IgM抗体阳性 18例 ,其中有 17例IgG、IgM抗体均为阳性 ,总阳性率为 4 1.5 % ;留观人员IgG抗体阳性 1例 ;其他均为阴性。 结论 :ELISA测定血清抗SARS冠状病毒抗体阳性病例可确诊感染病毒 ,对于观察期后 (一般为 2 1d)人员 ,阴性结果可以排除感染。Objectives: To provide basis for clinically diagnosing and excluding SARS patients, and to primarily investigate the possibility of infection in observed persons, physicians and nurses closely associated with SARS patients. Methods: The serum levels of anti-SARS coronavirus IgG and IgM antibody in 53 SARS patients, 29 suspected Persons, 63 observed persons and 114 physicians and nurses closely associated with SARS patients were measured by ELISA. Results:In clinically diagnosed SARS patients, the positive cases of IgG antibody were 21, the positive cases of IgM antibody were 18, in which both positive cases were 17, and general positive rate was 41.5%. In observed persons there was one whose serum level of IgG antibody was positive the others were negative. Conclusion: Cases with positive reaction could be diagnosed as patients already infected by the SARS coronavirus, and the cases with the negative results could be excluded infection after 21 days of observing.

关 键 词：SARS 酶联免疫吸附测定 严重急性呼吸综合征 冠状病毒 IGG抗体 IGM抗体 

分 类 号：R563.1[医药卫生—呼吸系统] R446.62[医药卫生—内科学]