从线粒体细胞色素b基因探讨矮岩羊物种地位的有效性  

The validity of the dwarf bharal (Pseudois schaeferi) species status inferred from mitochondial Cyt b gene

作　　者：周材权[1] 周开亚[2] 胡锦矗[1] 

机构地区：[1]西华师范大学珍稀动植物研究所,四川南充637002 [2]南京师范大学生命科学学院遗传资源研究所,南京210097

出　　处：《动物学报》2003年第5期578-584,共7页ACTA ZOOLOGICA SINICA

基　　金：四川省教委青年科学基金 (No .2 0 0 2B17)项目~~

摘　　要：用采自四川省的岩羊和矮岩羊共 18个头骨或皮张标本 ,分析了线粒体Cytb基因114 0bp的全序列 (其中一个样品只测到 80 2bp的基因片段 )。用NJ、MP、ML等系统发育分析法分别重建的系统发育树的拓扑结构完全一致 ,均不支持矮岩羊是单系群。结果提示 :所研究的全部样品都属于同一个物种 ,即岩羊P .nayaur ,不支持矮岩羊的物种地位。根据基于 17个样品Cytb基因全序列的系统发育树和遗传变异以及地理分布 ,这些岩羊样品聚为 5支 ,根据地理区划和本文分析结果 ,四川的岩羊可分为摩天岭、川西、川西北、川西南和川北 5个种群。基于 17个样品Cytb基因1137bp的编码区 ,共定义了岩羊的 16种单元型 ,在 5个种群间未发现共享的单元型.We reconstructed a molecular phylogeny of blue sheep (bharal) and dwarf blue sheep using complete sequences of the mitochondrial cytochrome b (Cyt b ) gene. Eighteen individuals of Pseudois collected from different localities in Sichuan Province, China, were sequenced by directly sequencing PCR products and phylogenetic trees were reconstructed from a branch and bound search of maximum parsimony, maximum likelihood method and the neighbor joining method. The results did not support the species status of P. schaeferi. According to our phylogenetic tree, genetic divergences and the geographic distribution, the blue sheep distributed in Sichuan Province were divided into 5 groups. According to the zoogeography, the 5 groups can be divided into 5 geographic populations, i.e. the Motianling population, the West Sichuan population, the North west Sichuan population, the South west Sichuan population and the North Sichuan population.

关 键 词：矮岩羊 有效性 Cyt6基因 线粒体细胞色素B基因 物种地位 

分 类 号：Q953[生物学—动物学]