长白山赤杨根瘤内Frankia的基因多样性分析  

Genetic Diversity of Frankia in Nodule of Alnus in Changbai Mountain Revealed by IGS PCR-RFLP

作　　者：代玉梅[1] 韩士杰[1] 唐晓萌[1] 张成刚[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110016

出　　处：《东北林业大学学报》2003年第6期6-8,共3页Journal of Northeast Forestry University

基　　金：国家重点基础研究发展规划资助项目(No .0 0 1CB1 0 89);中国科学院长白山森林生态系统定位研究站资助项目;中国科学院沈阳应用生态研究所创新项目(C1 1 -AG -SCXZY0 1 0 6 )

摘　　要：采集长白山自然保护区北坡东北赤杨、西伯利亚赤杨、色赤杨根瘤样品 2 1个 ,对根瘤内FrankiaDNA的 16S -2 3SrDNA和nifD -nifK两个基因间隔区段 (IGS)进行PCR -RFLP分析 ,研究其基因多样性。结果表明 :与赤杨共生的Frankia菌存在丰富的基因多样性 ,基因类型与宿主种型关系密切。东北赤杨对Frankia的特异性较高 ,与西伯利亚赤杨和色赤杨共生的Frankia菌有较近的亲缘关系。nodule samples were collected from Alnus mandshurica, Alnus sibirica, and Alnus tinctoria growing in Changbai Mountain. DNA was extracted directly fr om nodules, and its two target DNA regions that encode16S-23S rDNA intergenic sp acer (IGS) and nifD-nifK IGS were studied by PCR-RFLP. The results indicated that rich genetic diversity existed in Alnus-infective Frankia in Chang bai Mountain, and the genotypes were closely related to Alnus species. Aln us mandshurica showed greater specialization towards Frankia, and Franki a strains that infected Alnus sibirica and Alnus tinctoria had more cl ose relationship. Above results suggest the co-evolution between Frankia and its host plants Alnus.

关 键 词：长白山 赤杨 根瘤 FRANKIA 基因多样性 协同进化 

分 类 号：S792.11[农业科学—林木遗传育种] Q939.13[农业科学—林学]