栉孔扇贝热休克蛋白70基因cDNA的克隆与分析  

Identification and Cloning of Heat Shock Protein 70 Gene from Scallop Chlamys farreri

作　　者：吴龙涛[1] 宋林生[1] 胥炜[1] 邱丽华[1] 李红蕾[1] 苏建国[1] 相建海[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学开放实验室,青岛266071

出　　处：《高技术通讯》2003年第11期75-79,共5页Chinese High Technology Letters

基　　金：863计划(2002AA626020);973规划(G1999012008);国家自然科学基金(40276045)资助项目

摘　　要：应用表达序列标签法,获得了栉孔扇贝HSP70基因cDNA的部分序列,然后利用锚定PCR技术克隆到了该基因的全长cDNA。克隆到的HSP70 cDNA全长2553bp,编码区全长1902bp,可以编码634个氨基酸。Blast分析表明该序列与其他生物的HSP70基因具有很高的相似性。在该cDNA序列编码的氨基酸序列中含有3个HSP70家族的特征模体,结合Blast分析的结果,可以确认所获得的cDNA序列是栉孔扇贝HSP70的编码序列。该基因的克隆为进一步深入研究栉孔扇贝的抗逆机理以及指导扇贝的遗传选育和遗传改良都具有重要的理论意义。Expressed sequence tagging method was used to identify the partial sequences of HSP70 from Chlamys farreri , then the full length cDNA was cloned through the approach of Anchored-PCR. The full length of HSP70 cDNA from Chlamsy farreri was 2553bp and encoded 634 amino acids. BLAST analysis revealed that the HSP70 gene from C. farreri shared high identity with the HSP70 genes from other organisms in nucleotides and amino acids respectively. Three signature sequences belonging to HSP70 family were detected in the cDNA sequence from Chlamsy farreri. The result indicated that the cDNA sequence cloned from C. farreri was a member of heat shock protein 70 family, it could be used in the study of the anti-stress mechanism in scallop, and the genetic breeding or improvement.

关 键 词：栉孔扇贝养殖 热休克蛋白 表达序列标签法 基因克隆技术 病害防治 

分 类 号：Q51[生物学—生物化学]