Investigation of Mixed Chiral Selectors of Different Metal Ion-L-Alanine Complex and β-Cyclodextrin on the Chiral Separation of Dansyl Amino Acids with Capillary Electrophoresis  

作　　者：郑志侠 屈锋 林金明 

机构地区：[1]Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences

出　　处：《Chinese Journal of Chemistry》2003年第11期1478-1484,共7页中国化学（英文版）

基　　金：ProjectsupportedbytheNationalScienceFundforDistinguishedYoungScholarsofChina (No .2 0 12 5 5 14 ) ,theNationalNaturalScienceFoundationofChina (Nos .2 0 2 75 0 43and 5 0 2 73 0 46)and 863Program (No .2 0 0 1AA63 5 0 3 0 )

摘　　要：Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtained by using either Mn(II)- L -alanine complex or β -CD alone. The effects of separation parameters,such as pH value of buffer solution,capillary temperature,the concentration of Mn(II)- L -alanine complex,the types of CD and ligand on the migration times and resolutions were investigated. Six different transition metal complexes, Cu(II), Zn(II),Co(II),Ni(II),Hg(II) and Cd(II)- L -alanine complexes have been employed and compared with Mn(II) complex. Differences in retention and selectivity were found. The substitution of Cu(II),Zn(II),Co(II) and Ni(II) for Mn(II) resulted in a better chiral resolution while Hg(II) and Cd(II) showed poorer resolution abilities. The chiral separation mechanism was also discussed briefly.Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtained by using either Mn(II)- L -alanine complex or β -CD alone. The effects of separation parameters,such as pH value of buffer solution,capillary temperature,the concentration of Mn(II)- L -alanine complex,the types of CD and ligand on the migration times and resolutions were investigated. Six different transition metal complexes, Cu(II), Zn(II),Co(II),Ni(II),Hg(II) and Cd(II)- L -alanine complexes have been employed and compared with Mn(II) complex. Differences in retention and selectivity were found. The substitution of Cu(II),Zn(II),Co(II) and Ni(II) for Mn(II) resulted in a better chiral resolution while Hg(II) and Cd(II) showed poorer resolution abilities. The chiral separation mechanism was also discussed briefly.

关 键 词：chiral separation ligand-exchange capillary electrophoresis dansyl amino acids Β-CYCLODEXTRIN 

分 类 号：O658.9[理学—分析化学]